FastQCFastQC Report
Sun 2 Jul 2023
SRR3494969.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3494969.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22486143
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG538977823.96933080075138TruSeq Adapter, Index 5 (100% over 49bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCG5226912.3245026948374385TruSeq Adapter, Index 5 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC1507050.6702127616995054TruSeq Adapter, Index 5 (100% over 50bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1460630.649568936744732No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGT1349000.599925029383652TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1039160.46213350150801763TruSeq Adapter, Index 5 (100% over 50bp)
TTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG892410.39687108633970714TruSeq Adapter, Index 5 (100% over 37bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA890200.3958882588267805Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT813430.3617472325067042Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGT783420.3484012353741591TruSeq Adapter, Index 5 (100% over 38bp)
AGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGA643530.2861895879609055Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC621420.27635686564832396No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTC597400.2656747313223081TruSeq Adapter, Index 5 (100% over 50bp)
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC573190.25490810051328056Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC539660.23999669485335923No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG410070.18236564625600754Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC376630.16749426524593392Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT372080.16547079683696755No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA342050.1521159053377896Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA312800.13910789413729158Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA300080.13345107695881858Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC275590.12255992501693154No Hit
GGTGCGGAGAGCCGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA275200.12238648486759156Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATCCGGCTCGAAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC272600.12123021720532508TruSeq Adapter, Index 5 (100% over 35bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC271870.12090557282322717No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG269960.12005616080979294No Hit
GGGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA259500.11540440706082854Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC250510.11140638925937632Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC244750.10884481166912441No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT243550.10831114967115527No Hit
GGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG239580.10654561789454065Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)