FastQCFastQC Report
Mon 10 Jul 2023
SRR3494974.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3494974.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53994102
Total Bases5.4 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT8780281.6261553900831611No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC6106221.1309050014388609No Hit
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG5292810.9802570658550818Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT5076200.9401397211865845No Hit
CCGTGGCGCAATGAAGGTGAAGGGCAGATCGGAAGAGCACACGTCTGAAC3017330.5588258510160979Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTC2955220.547322742769201Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA2914920.5398589645957997Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TACCCGTCGCCGCGCTCTCCCTCTCCCCAGATCGGAAGAGCACACGTCTG2907020.5383958418273166Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC2884530.534230572072483No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA2883420.5340249940632404Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG2637000.48838667601139096TruSeq Adapter, Index 1 (100% over 49bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG2415120.44729329881252583No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC2302560.42644657744284736No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT2241400.41511941433899574No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCTG2003930.37113868474004813Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACT1988900.3683550473716555Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGGGGTCCGGTGCGGAGAGCCGTTCAGATCGGAAGAGCACACGTCTGAA1853320.34324489737786545Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG1828320.3386147620345645No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC1820740.33721090499847556No Hit
AGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGA1767640.3273764975293042Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT1723960.31928672505748873No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT1677890.3107543116468536No Hit
TTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAAC1625540.30105880823798126Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC1459330.2702758164215788No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG1362450.2523331159392187Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCGTCGCCGCGCTCTCCCTCTCCCCAGATCGGAAGAGCACACGTCTGA1320660.24459338169935674Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1301230.24099484051054318Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG1275310.2361943161866087No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCAGATCGGAAGAGCACACGTCTGA1229860.22777673013248742Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1202690.2227446990413879Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC1039910.19259696179408634No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1023100.18948365878925072Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TACCCGTCGCCGCGCTCTCCCTCTCCCCCAGATCGGAAGAGCACACGTCT1007500.1865944543350309Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT985840.18258290507359487No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA985610.18254030782843653Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC921470.17066123259166344No Hit
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTCT884710.16385308158287362Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGCCCGTCACGTTGAACGCACGTTCAGATCGGAAGAGCACACGTCTGA856480.15862473275321812Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC835620.15476134782276774Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAGATCGGAAGAGCACACGT826010.15298152379680285No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG802820.14868661025235683Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTAGATCGGAAGAGCACACGTC782350.14489545543326193No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT761700.14107096363969532No Hit
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA710660.13161807932281197Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG699880.12962156496278057Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA683920.12666568655961719Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC679070.1257674403030168Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA674650.12494883237432117No Hit
AATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA649200.12023535459484075Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC629140.116520133995376No Hit
CCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAAC616480.11417543345752837Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC613950.11370686376078631No Hit
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA608560.11270860658077062Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC604890.11202890271237403Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GACCGGGGTCCGGTGCGGAGAGCCGTTCAGATCGGAAGAGCACACGTCTG594090.11002868424406799Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGACCGGGGTCCGGTGCGGAGAGCCGTAGATCGGAAGAGCACACGTCT579560.10733764958254144Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC567330.10507258737259859Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC559370.10359835227929154Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA549860.10183704879469983Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC542800.10052949857375162Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)