FastQCFastQC Report
Tue 4 Jul 2023
SRR3494975.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3494975.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65697897
Total Bases6.6 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT10998711.6741342572959375No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC7513221.1436012936608915No Hit
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG6401330.9743584334213925Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT6229700.9482343095396188No Hit
CCGTGGCGCAATGAAGGTGAAGGGCAGATCGGAAGAGCACACGTCTGAAC3733150.5682297562736293Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TACCCGTCGCCGCGCTCTCCCTCTCCCCAGATCGGAAGAGCACACGTCTG3636810.5535656643621333Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC3588670.546238184762596No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA3581170.5450965957099053Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTC3576740.5444222971094493Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA3508560.5340444915611225Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG3153630.48001993123158876TruSeq Adapter, Index 1 (100% over 49bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG2960910.4506856589336489No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC2817580.4288691310773616No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT2757000.4196481357690947No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCTG2457040.3739906621364151Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACT2437120.3709586016124687Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGGGGTCCGGTGCGGAGAGCCGTTCAGATCGGAAGAGCACACGTCTGAA2283800.3476214771379973Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG2274150.3461526325568686No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC2270820.34564576701747396No Hit
AGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGA2128340.32395861925382485Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT2125180.3234776297329578No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT2072580.3154712851767538No Hit
TTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAAC2015110.30672366879566937Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC1806510.2749722719434992No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCAGATCGGAAGAGCACACGTCTGA1672670.25460023476854976Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG1664570.2533673185916438Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1574860.23971239140272632Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG1557850.23712326743122383No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCAGATCGGAAGAGCACACGTCTGA1548290.2356681219187275Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1449680.2206585090539504Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC1271020.19346433570012142No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCAGATCGGAAGAGCACACGTCT1255870.19115832581368625Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1235340.1880334160467876Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT1232110.18754177169476216No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1207990.18387042130130893Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC1125320.17128706570318378No Hit
CTCGCCCGTCACGTTGAACGCACGTTCAGATCGGAAGAGCACACGTCTGA1073270.16336443767751044Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTCT1069610.1628073422197974Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC1016190.1546761839271659Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAGATCGGAAGAGCACACGT1010510.15381162048459482No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG988080.15039750815768121Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTAGATCGGAAGAGCACACGTC954930.1453516845447884No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT931130.14172904195091665No Hit
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA874580.13312146049362888Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA852260.12972409147282143Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG849320.12927658856416668Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC831610.12658091628107976Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA822010.12511968229363565No Hit
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA784700.11944065728618375Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA782280.1190723045518489Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC776310.11816359966590712No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC763230.11617266835801457No Hit
CCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAAC746420.11361398676125052Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC738550.11241607931529375Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GACCGGGGTCCGGTGCGGAGAGCCGTTCAGATCGGAAGAGCACACGTCTG727340.11070978421120542Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGACCGGGGTCCGGTGCGGAGAGCCGTAGATCGGAAGAGCACACGTCT709210.1079501829411678Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC695710.10589532264632458Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA678940.10334272952450822Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC674680.10269430694257992Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCCGTCGCCGCGCTCTCCCTCTCCCCCAGATCGGAAGAGCACACGTCTG663220.10094995887006855Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC659710.10041569519340932Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)