FastQCFastQC Report
Sun 2 Jul 2023
SRR3494977.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3494977.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70163397
Total Bases7 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG7081411.0092741091198878TruSeq Adapter, Index 12 (100% over 49bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC5062890.7215856438649912No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA3612360.5148496444663305Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC3540750.5046434681604711No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA2935350.41835916239916376Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG2747630.39160447148817495No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2593750.36967280817375475No Hit
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC2502330.356643222391299Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT2023060.2883355262858781Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG1559210.22222555729449642Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGA1469810.2094838709134907Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC1439020.20509554290822035Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1415710.20177329783505207Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA1157890.16502764254701066No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC1084250.1545321415951397Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1022820.14577686425302355Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA1013670.14447276547912868Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1013380.14443143338684128Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC925920.1319662444507925No Hit
AGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAA878920.12526759501111384Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC868660.12380529409087762Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT831250.11847345418580574No Hit
AGTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC826670.11782069217657748Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTC807940.11515120911263746No Hit
ACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA786120.11204132547915262Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC774870.11043792534731466No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG774510.11038661654309583Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG772530.11010441811989236Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC771400.10994336548442772Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA749810.10686626247586047Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA712280.10151731963604899No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)