FastQCFastQC Report
Sun 2 Jul 2023
SRR3494979.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3494979.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33712338
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT3564311.0572716730592817No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC2740990.8130524794809545No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA2697180.8000572372049662Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG2288380.6787959945109711TruSeq Adapter, Index 10 (100% over 49bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC2214200.6567921809516741No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC1709590.5071110760695388No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC1633410.4845140078982359No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG1500050.444955790369686No Hit
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT1437590.4264284488367434Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC1391160.41265604301902764No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT1325830.3932773811178566No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1181160.3503643087584136Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA1135400.33679064323571983Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG953850.282937955830889Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTC915730.2716305229260575Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG894950.26546660750731677Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA850640.2523230515783272No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT810780.24049948716105066No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG810530.2404253303345499Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACCCGTCGCCGCGCTCTCCCTCTCCCCAGATCGGAAGAGCACACGTCTG798000.2367085901903333Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT755100.22398327876280785Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG697860.20700433176720048No Hit
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA666110.19758641480160763Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC639480.18968722964274978No Hit
GGGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA637700.1891592330380646Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACT599330.1777776433067324Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA597200.17714582714494617Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT584810.17347061482356993No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC552560.16390438420497563Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC544460.16150170302635197No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT512340.15197403395753803No Hit
AGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGA490560.14551349123279436Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAAC485050.14387907477671824Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT474890.14086534134772855No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC473170.1403551423814035No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT472530.14016530090556165Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC463330.13743632969033473No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC444480.13184490497217963Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT424510.12592125767130122Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC417490.1238389339831607Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT417260.12377070970278004No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA414010.12280667095827053No Hit
AGTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC386280.11458119576280945Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA378900.11239208624450787Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTC354580.10517811016251676No Hit
ACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC347970.10321740366983745Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG347050.10294450654831475Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)