FastQCFastQC Report
Sun 2 Jul 2023
SRR3494985.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3494985.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31735354
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG10169393.204435658729378TruSeq Adapter, Index 3 (100% over 49bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA5422341.70861178986691No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC5234391.6493876198765578No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC5130271.6165787846576407Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC4918961.5499937388440665No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT3402551.0721638712459296Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA3067960.9667325595296653No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT2313960.729142646399974Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC1712730.5396914746878197Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT1666850.5252344120692651Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT1526830.48111327196791315Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1462410.4608141443766469Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC1399490.4409876757637554Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC1363460.4296344071031947No Hit
GGGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1314990.4143612199819797Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTCT1089850.3434182583877905Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGT932700.29389935275339923Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT923120.2908806374115127Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC914280.28809510049895776Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG833290.2625746667265788Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA816670.25733760524618693Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT796250.25090314102057915Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC770150.24267887479685904No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG768700.24222197111776347No Hit
GTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACTCCAG745310.23485164211497372Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT718690.22646352077875043Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT661170.20833862448800794No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT596870.1880773096150117No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC592460.18668769221859002No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT583680.18392106166517003No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTCT574800.1811229205131917Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC572310.18033830660908967No Hit
AATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC562250.1771683403941232Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC560150.1765066178243986No Hit
GTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACTC550560.17348475142265626Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC531270.1674063569607574Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT514650.16216929548036552No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC508200.16013686187335424No Hit
ATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCTGAACTC507300.15985326648632942Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGTC484280.15259952669820542No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCTG458950.14461789208338435Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCAGATCGGAAGAGCACACG456460.14383327817928232No Hit
TCCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTC455220.14344254675715923No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC446120.14057508228835258No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGTCT445750.14045849307368685Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG445230.1402946379611836Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT444890.14018750192608534Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGGGGTATGATTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAG444230.13997953197560045Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA413980.13044757591171033Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG389300.12267076018751832Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCA379990.11973712346173923No Hit
GGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCA376800.118731935367729Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC349170.1100255569860667Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC342270.10785132568554301Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG337650.10639553603214887No Hit
GTCTAGGGGTATGATTCTCGGAGATCGGAAGAGCACACGTCTGAACTCCA321840.10141371040007935Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG320310.10093159824213714Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)