FastQCFastQC Report
Sun 2 Jul 2023
SRR3494988.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3494988.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45626606
Total Bases4.6 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG11508342.522287105904831TruSeq Adapter, Index 10 (100% over 49bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT3822770.837837905366005Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC3661950.8025909268815655No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT3366270.7377866326502568Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA2675940.5864867529265709Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC1799930.3944913193850097No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA1399140.3066500278368284No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC1370000.3002634033309425Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1355980.2971906347800667Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG1351350.29617587597902856No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1343600.2944773056317185No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC1333230.2922045089218339No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG1177510.25807529931110806Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1006740.22064757567108984Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA874760.1917214705823177Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT860620.18862240158735455Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC833440.18266535100156256No Hit
AGTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC762580.1671349387679636Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AACCCGGCGGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA739180.16200635217092413TruSeq Adapter, Index 10 (100% over 35bp)
AATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA724070.15869468791958796Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAGATCGGAAGAGCACACG720500.15791224970798837No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA709000.1553917904829476No Hit
GTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACTCCAG679520.14893064805214748Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC679210.14886270523825507Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA648610.1421560919959727Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTCT645850.1415511817819629Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCACCCAGGGACGCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC639760.1402164342445283Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGGCCCGGTGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCT630450.13817595812408226Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT629800.1380334973852756No Hit
AGTCCGCCCGGAGGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA567580.12439671712596813Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC563200.12343675091677868TruSeq Adapter, Index 10 (100% over 37bp)
AATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC558020.12230144841367337Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC546490.11977441407761077Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACTC539150.11816570358093258Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT524480.11495047429125015Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT518700.11368366956770794No Hit
ACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA517170.11334833890559382Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA508430.11143278989456283Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC500010.10958737540109821No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)