FastQCFastQC Report
Sun 2 Jul 2023
SRR3494989.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3494989.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27263518
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG3388621.2429136988117233TruSeq Adapter, Index 9 (100% over 49bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC3004621.1020661383464894No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT2601140.9540734985118208Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1949760.7151534882622265Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC1897180.6958676426131066No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1738030.6374929310296639Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1721330.6313675293115144No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG1288080.47245553563556986No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA1100660.4037116559939183No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1049950.3851117086210224Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC1019570.37396861256129893No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT949480.3482602648711733No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC764760.2805067196390429No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA647420.2374675197822966No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG635880.2332347571578987No Hit
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC511950.18777840776087665Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT501470.1839344430898463No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC478840.17563397357597063No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT466390.17106743157651189Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC437170.16034981252236047Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC427560.15682495560550916No Hit
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG413910.15181826497959655Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC399160.14640810477943456TruSeq Adapter, Index 9 (100% over 37bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA340690.12496186295547038Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT339080.12437133021497813No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC305770.11215353792566314No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA294490.1080161408369969Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC285630.10476637681167925No Hit
ACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA274670.10074635269006736Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)