FastQCFastQC Report
Thu 6 Jul 2023
SRR3494991.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3494991.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30403193
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG7611922.503658086175357TruSeq Adapter, Index 6 (100% over 49bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC4976631.6368774161319175No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA2401930.7900255739586298No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT2226580.7323507106638437Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA2184040.7183587592263747Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1593650.5241719183902822Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC1516620.498835763730474No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC1468520.48301505700404557Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG1311740.4314481048092548Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT1303300.42867208059364026Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA1284750.4225707477500801No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG1180250.3881993578766546No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT1119630.36826066262185025No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT940270.3092668589118255No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC939770.30910240250094784No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT764610.2514900326422952Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC643440.2116356660302094No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC622190.20464626856790996No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC609430.20044934096231276Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA598730.19692997376953136Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG597190.19642344802402825Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA573740.18871044235386725Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTCT567510.18666131547433193Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC545720.17949430508828462Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAGCACTGAAAATGCTTAGATGAGATCGGAAGAGCACACGTCTGAACTC514820.16933089889604688Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC490020.16117386091651623No Hit
GGGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA481120.15824653680289436Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACTC457120.15035262908076794Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT448280.14744503973645137Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC403010.1325551562955904Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG391440.12874963494788194No Hit
GTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACTCCAG381610.12551642191002768Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT334520.11002791713357213No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT330270.10863003764111225No Hit
ATCTGGCCTACACCCAGAAGATTAGATCGGAAGAGCACACGTCTGAACTC316600.10413379936771773Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGTCTG311800.10255501782329243Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC304290.10008488253191038No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)