FastQCFastQC Report
Sun 2 Jul 2023
SRR3494993.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3494993.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39961922
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG14999413.753425573474669TruSeq Adapter, Index 5 (100% over 49bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC5128961.2834617914523732No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC3324650.8319544790663472No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA2635160.659417732710654No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT2548910.6378346867300326Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG2312520.578680875259203No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC1426730.35702236744268706No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1347770.33726355804408004Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1279750.32024235470956575Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1229920.3077729844925877No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC1017350.25457984728562355No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC1016450.25435463289278226No Hit
GCCCACCCAGGGACGCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1003490.25111154563586807Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT951260.23804160370464664No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG924130.23125264095155385Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC810780.2028881393642678Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAGATCGGAAGAGCACACG799890.20016304521088849No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA715080.17894034225881328Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCG706740.17685335555181755TruSeq Adapter, Index 5 (100% over 50bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC704810.17637039579828018No Hit
ACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTG697460.17453114492340982Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC689310.17249170347712503No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT684810.17136563151291873No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG645200.16145369584576036Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCCCGGTGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCT614130.15367879452845137Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT588810.14734276294318377No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA559500.14000828088298656Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA557360.1394727711044529Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACG522590.130771988394352No Hit
GCCCGGTGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGA516050.1291354304730388Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG508920.12735123200530746Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC503600.1260199647054013TruSeq Adapter, Index 5 (100% over 50bp)
CGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGTCTG474220.11866796597020533Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGAGATCGGAAGAGCACACGTCTGA455590.11400602803839115Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA453270.11342547538128922Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC442790.11080297889575982Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC441400.1104551477779272Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTC429570.1074948297031359No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC422010.10560302880326927No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)