FastQCFastQC Report
Thu 6 Jul 2023
SRR3494994.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3494994.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31215018
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG11866603.8015675659709696TruSeq Adapter, Index 5 (100% over 49bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC3941441.2626742678796468No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC2563810.8213386261702621No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA2009190.6436613299406074No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1956440.626762412887284Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG1793150.5744510542970054No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC1086740.3481465235740053No Hit
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1041190.333554188563979Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1022730.3276403684918586Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT941530.30162724878133984No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC812440.2602721548967231No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC812020.2601376042775308No Hit
GCCCACCCAGGGACGCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC798440.25578713425697847Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT755500.24203093523764746No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG742090.2377349261820064Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAGATCGGAAGAGCACACG626850.2008167991445656No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC621930.19924063474831252Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCG554550.17765487112645587TruSeq Adapter, Index 5 (100% over 50bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA546350.17502793046603402Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC546110.1749510443979241No Hit
ACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTG543700.17417898013065378Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC541780.1735638915857745No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT534160.17112275892328493No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG485690.1555949767512548Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCCCGGTGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCT480570.15395474063157674Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT458200.1467883183664991No Hit
GGGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA433920.1390100111427134Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA432370.13851345528617026Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACG410660.13155846970839485No Hit
GCCCGGTGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGA410490.13150400874348367Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC390690.12516090812441627TruSeq Adapter, Index 5 (100% over 50bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG380360.12185160360951898Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGTCTG369140.1182571799253808Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGAGATCGGAAGAGCACACGTCTGA363680.11650802187588039Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA353570.11326919625675053Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC349140.11185000758288847Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC345920.1108184528357472Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTC340200.1089860015457944No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC318900.10216236300104008No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)