FastQCFastQC Report
Sun 2 Jul 2023
SRR3494995.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3494995.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31318840
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG4676891.4933152057994485TruSeq Adapter, Index 12 (100% over 49bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC4416871.4102916966273336No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC3314081.058174568406748No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA2862890.914111122889609Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2706110.8640517975761555No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT2056920.6567676197458143Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG2001000.6389125523167525No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1665400.5317566040121537Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC1146190.36597460186903474No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA1126180.3595854763458672No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG991000.3164229581938539Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC945250.30181513746997013No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA925360.29546432754214397No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG843410.26929796889029095Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTCT790830.25250935219822956Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC680460.21726858338303717No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC672120.21460564950681443Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTC644260.20571004545506794No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG599660.1914694158532053No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT541450.1728831591463796No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT493640.15761758736913628Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA491740.15701092377623183Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC481220.15365192325130816Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACACG472330.15081337622977095No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC445400.14221471804191982No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT441010.14081300584568268Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAGATCGGAAGAGCACACG425920.13599481973150984No Hit
GTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACTCCAG404480.12914910003052477Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCT384060.122629062889941Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT379700.12123692959253919Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACTC372850.11904974769180467Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTG371260.118542066053532Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCACCCAGGGACGCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC346390.11060115891904042Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG341830.10914516629606973Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC341350.10899190391470438Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGTCTG323090.10316154749026465Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT318010.10153952062081481No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)