FastQCFastQC Report
Sun 2 Jul 2023
SRR3494996.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3494996.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44664518
Total Bases4.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG6692621.498419841897768TruSeq Adapter, Index 12 (100% over 49bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC6246901.3986269817128667No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC4818441.0788071193335165No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA4010040.8978133380953536Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT3880440.8687970169072462No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT2984290.6681567681979687Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG2850070.6381060688934335No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA2315680.5184607611796013Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC1656240.37081783799838614No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA1596300.3573977894488865No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1386040.31032239058305744Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC1354420.3032429455524405No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA1270910.2845457774782211No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG1197430.26809423981693925Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTCT1137990.2547861369510357Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC981720.2197986329999128No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC952350.21322294354547833Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTC921380.20628902790353634No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG863500.19333019557045258No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT784900.17573233410914676No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT689230.15431264700987032Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC688070.1540529330239274Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA684910.15334543630360012Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACACG680600.1523804645110018No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC633180.14176353587874832No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT615760.13786334826226043Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAGATCGGAAGAGCACACG613840.13743347683725143No Hit
GTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACTCCAG574350.128592006746832Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCT547690.12262306289748832Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT546640.12238797696193654Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTG532530.1192288697708548Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACTC521080.11666531361650427Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG486830.10899703429017189Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC480140.10749920104365616Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCCACCCAGGGACGCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC470790.10540581676040923Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGTCTG463340.10373782607482745Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT456390.10218178107284175No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)