FastQCFastQC Report
Sun 2 Jul 2023
SRR3495002.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3495002.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15116803
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC3563812.3575156731221543No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG3263812.1590610130991323TruSeq Adapter, Index 9 (100% over 49bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1596751.0562749279725347Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC1353490.8953546593152004No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA1299710.8597783539284066No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA1208010.7991173795147029No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1169220.7734571919737262Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG1064940.7044743521497236No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT970150.6417692947377828No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG854780.5654502476482627Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT690490.4567698606643217No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC625280.41363243273065076No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC591340.3911805955267129Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG574130.3797959131967255No Hit
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTCT494230.32694082207726066Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT453560.30003698533347295Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC424920.28109118045660847Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC372690.24654022414660032No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG322610.21341152623342383Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT311260.2059033249292195No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT300670.19889787543040682Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA273990.18124864099902604No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC267300.17682310208051266No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC259120.17141190501721826No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG249330.16493566794513365No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC246250.1628982001022306No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG241460.15972954069719636No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC233420.15441095580857936No Hit
GCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAGATCGGAAGAGCACACG230810.15268440026637908No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA223510.14785533687248553Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC214830.1421133820424861Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACAC209270.13843535567672607No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA206550.1366360334258507Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTC193630.12808925273419253No Hit
AGGCCCGGTGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCT179360.1186494260724308Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT175730.11624812468615223No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACACG164840.10904422052731652No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACACG161310.10670907069437896No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGTC158220.10466498769614184No Hit
AAGGCCCGGTGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTC158070.10456576036613031No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGTCT157540.10421515713342297Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG156620.10360656284268573No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)