FastQCFastQC Report
Tue 4 Jul 2023
SRR3495003.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3495003.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34407695
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG10132042.944701759301226TruSeq Adapter, Index 6 (100% over 49bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC6477121.8824626293624143No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT3118530.9063466762304188No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC3039730.8834448224445143No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA2806600.815689629892383Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG2060120.5987381601702759No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA1989840.5783124966668066No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1804750.524519297209534Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1477410.42938360154610766Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC1170210.34010124770055067No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG1122470.32622644440436943Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA1072000.3115582139402247No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1038070.30169704770982186No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA999470.29047862694667576Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTCT854820.24843861235110343Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT823550.23935052900230605Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC773530.22481308323617724No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT727010.21129285178795032Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTC721530.2097001847987783No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT712050.20694498716057555No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT678560.19721169930156612No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG660260.19189312158225072No Hit
CGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGTCTG652540.18964943742962148Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT643560.18703955612254758No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC642940.18685936387194785No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG625810.1818808263674739No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG619160.1799481191634604No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC611740.17779162480950844No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC600870.17463244777076756No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGT595570.1730920946608019No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG551420.16026066262212565Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA542630.1577060015208807Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC537680.15626736984270523Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG524290.152375798495075Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC505920.14703687648940156No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT484090.1406923654723166Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG477550.13879162786115143Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC465770.13536797509975604No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACACG460380.13380146505018717No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG459300.1334875817749489Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTG452940.1316391580429901Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGTC451390.1311886774164907No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG447810.13014821248560823No Hit
ATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCT396250.11516319241960266Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA394390.11462261566780338Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCT378660.1100509638904902Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA374010.10869952201099202No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC371200.10788284423004796No Hit
TCCGGCTTTCTGGAAGAATTCACTGAGAAAGATCGGAAGAGCACACGTCT353310.1026834259022582Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)