FastQCFastQC Report
Tue 4 Jul 2023
SRR3495004.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3495004.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31499091
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG4133271.3121870723190077TruSeq Adapter, Index 5 (100% over 49bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC2130360.6763242786910898No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC1945920.6177702080355272No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1829430.5807881884591527Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1743380.5534699398150886Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC1449440.4601529612394212Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1311890.4164850344411526No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1202340.38170625304711175Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1171820.37201708455650356No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC1098560.3487592705452992No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT1010690.3208632274499604No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA866280.27501746002765604No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC825090.2619408921990796No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG793820.25201362159942964No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT747190.2372100198066033No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC716380.2274287851671656No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA691950.21967300580197696No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC670000.2127045507440199No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA558870.1774241675735976Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT544070.1727256192885058Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTCT536340.17027158021798153Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG524140.16639845257756802Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT502330.1594744432466321No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC466630.14814078285624177Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG430620.13670870692744752No Hit
ATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCT428200.13594043078893928Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC406230.12896562634140776No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG405430.12871165075842983No Hit
GTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACTC403540.12811163344364446Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT368160.11687956328644532No Hit
ACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTGA365580.11606049203134149Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG363440.1153811073468755No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA352940.11204767782029013Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC329180.10450460300584546Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGA316560.10049813818436856Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)