FastQCFastQC Report
Sun 2 Jul 2023
SRR3495992.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3495992.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12934604
Total Bases646.7 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGAGATCGGAAGAGCACACG5874094.541375986462361No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG3794012.933224704830546TruSeq Adapter, Index 1 (100% over 49bp)
CTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC1802781.39376512802402No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAAGATCGGAAGAGCACACGT1551881.1997893402844029No Hit
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCTAAGATCGGAAGAGCACA933710.7218697998021432No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGTAGATCGGAAGAGCACAC878560.6792322362555514No Hit
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAAC677890.5240902620598203Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCAGTAAAACGCGGCGTGTTCGAATTCAGATCGGAAGAGCACACGTCT521190.4029423707134753Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGGTGCGTGCCGCACTCACGAGGGACTGCCAAGATCGGAAGAGCACACG447890.34627268063251104No Hit
GAATGCATTGGATGGATGCCCGGGCATTGAGAGATCGGAAGAGCACACGT422620.3267359402730845No Hit
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCTAGATCGGAAGAGCACAC369920.2859925205286532No Hit
AACCGGGGTTCGAATCCCCGTAGGGACGCCAAGATCGGAAGAGCACACGT367460.2840906455273003No Hit
ACCGGGGTTCGAATCCCCGTAGGGACGCCAAGATCGGAAGAGCACACGTC348390.2693472486672186No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTAGATCGGAAGAGCACACGTCTG288780.2232615702807755Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGTGCGTGCCGCACTCACGAGGGACTGCCAGAGATCGGAAGAGCACAC287840.22253483755668127No Hit
GGGTACACGGAGTCAAGACCCGCAACGCGTAGATCGGAAGAGCACACGTC240350.1858193725915382No Hit
TCCGTTGGTTCGAATCCAGCTACCCCTGCCAAGATCGGAAGAGCACACGT224180.17331802349727907No Hit
GGGTACACGGAGTCAAGACCCGCAACGCGAAGATCGGAAGAGCACACGTC215420.16654549300465635No Hit
ACGTGTCGGTTCGAGTCCGACCCTCGGGACCAAGATCGGAAGAGCACACG214550.16587287867490957No Hit
GGGAGAGTGACACCCAGGCAGACGTGCCCTCAGATCGGAAGAGCACACGT186950.14453476890363245No Hit
CTGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACGT164210.12695402193990632No Hit
CCGGGGTTCGAATCCCCGTAGGGACGCCAAGATCGGAAGAGCACACGTCT161440.12481247976358611Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTACACGGAGTCAAGACCCGCAACGCGGAGATCGGAAGAGCACACGTC154550.11948568351995933No Hit
GGGTACACGGAGTCAAGACCCGCAACGCAGAGATCGGAAGAGCACACGTC153710.11883626278779001No Hit
GACGTGTCGGTTCGAGTCCGACCCTCGGGACCAAGATCGGAAGAGCACAC138670.1072085392022825No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCAGATCGGAAGAGCACACG137570.10635810729110841No Hit
TCGGGAGTTCGAATCTCTCTGAGCGCACCAAGATCGGAAGAGCACACGTC134020.10361353157777385No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)