FastQCFastQC Report
Sun 2 Jul 2023
SRR3495993.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3495993.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6104225
Total Bases305.2 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTGCGTGCCGCACTCACGAGGGACTGCCAAGATCGGAAGAGCACACGT3642845.967735461913675No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGAGATCGGAAGAGCACACG3331385.457498699671129No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG2191013.5893336172896637TruSeq Adapter, Index 2 (100% over 49bp)
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCTAAGATCGGAAGAGCACA798721.308470772292961No Hit
CTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC777071.2730035344372135No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGTAGATCGGAAGAGCACAC627101.0273212406161307No Hit
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCTAGATCGGAAGAGCACAC399060.6537439232662623No Hit
GAATGCATTGGATGGATGCCCGGGCATTGAGAGATCGGAAGAGCACACGT286770.4697893672005865No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTAGATCGGAAGAGCACACGTCTG283190.4639245768299825Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAAC214540.35146148774004893Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCAGTAAAACGCGGCGTGTTCGAATTCAGATCGGAAGAGCACACGTCT191590.3138645774033559Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACCGGGGTTCGAATCCCCGTAGGGACGCCAAGATCGGAAGAGCACACGT148490.24325774361200644No Hit
ACCGGGGTTCGAATCCCCGTAGGGACGCCAAGATCGGAAGAGCACACGTC138390.22671182664466008No Hit
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTAAGATCGGAAGAGCACAC128130.20990379614119728No Hit
TCCGTTGGTTCGAATCCAGCTACCCCTGCCAAGATCGGAAGAGCACACGT110760.18144809537656295No Hit
ACGTGTCGGTTCGAGTCCGACCCTCGGGACCAAGATCGGAAGAGCACACG100230.1641977482809038No Hit
AATGCATTGGATGGATGCCCGGGCATTGAGAGATCGGAAGAGCACACGTC95010.15564629416510697No Hit
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTAGATCGGAAGAGCACACG91730.15027296667472118No Hit
GGGAGAGTGACACCCAGGCAGACGTGCCCTCAGATCGGAAGAGCACACGT88150.14440817630411723No Hit
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCAGATCGGAAGAGCACACG86500.14170513046291708No Hit
GGGTACACGGAGTCGACCCGCAACGCGAAGATCGGAAGAGCACACGTCTG83930.13749493178904776Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGTGCGTGCCGCACTCACGAGGGACTGCCAAGATCGGAAGAGCACACG83200.13629903878051677No Hit
GGGTACACGGAGTCAAGACCCGCAACGCGTAGATCGGAAGAGCACACGTC76300.12499539253549795No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC75120.12306230520663967TruSeq Adapter, Index 2 (100% over 50bp)
GGGTACACGGAGTCAAGACCCGCAACGCGAAGATCGGAAGAGCACACGTC74850.12261998861444326No Hit
GGGTACACGGAGTCGACCCGCAACGCGTAGATCGGAAGAGCACACGTCTG73470.12035925936543951Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACGT70870.11609991440354836No Hit
CTCATTAAATCAGTTATAGTTTGTTTGATGAGATCGGAAGAGCACACGTC70640.11572312619538107No Hit
CCGGGGTTCGAATCCCCGTAGGGACGCCAAGATCGGAAGAGCACACGTCT69370.11364260000245731Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGTACACGGAGTCGACCCGCAACGCTGAGATCGGAAGAGCACACGTCTG63550.10410822012622405Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC63130.10342017209391856TruSeq Adapter, Index 2 (100% over 50bp)
TCGGGAGTTCGAATCTCTCTGAGCGCACCAAGATCGGAAGAGCACACGTC62590.10253553890952578No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)