FastQCFastQC Report
Sun 2 Jul 2023
SRR3495994.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3495994.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8076841
Total Bases403.8 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG3941044.87943244147062TruSeq Adapter, Index 3 (100% over 49bp)
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGAGATCGGAAGAGCACACG3188573.9477934504343963No Hit
CTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC1554791.9249976568809513No Hit
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCTAAGATCGGAAGAGCACA1034831.2812311149866638No Hit
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCTAGATCGGAAGAGCACAC858831.0633241387319623No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGTAGATCGGAAGAGCACAC573450.7099929291662421No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAAGATCGGAAGAGCACACGT486390.6022032623893425No Hit
GAATGCATTGGATGGATGCCCGGGCATTGAGAGATCGGAAGAGCACACGT461060.5708419913181403No Hit
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTAAGATCGGAAGAGCACAC295890.3663437227500207No Hit
AACCGGGGTTCGAATCCCCGTAGGGACGCCAAGATCGGAAGAGCACACGT248190.3072859797537181No Hit
ACCGGGGTTCGAATCCCCGTAGGGACGCCAAGATCGGAAGAGCACACGTC247490.30641930427997777No Hit
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCAGATCGGAAGAGCACACG227360.2814961938708463No Hit
CTCATTAAATCAGTTATAGTTTGTTTGATGAGATCGGAAGAGCACACGTC226360.2802580860512173No Hit
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTAGATCGGAAGAGCACACG214270.2652893625119028No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTAGATCGGAAGAGCACACGTCTG210020.26002740427847965Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGTTGGTTCGAATCCAGCTACCCCTGCCAAGATCGGAAGAGCACACGT189660.2348195290708335No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTCAGATCGGAAGAGCACACGTCT187850.23257855391730503Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAACT178480.22097748364738146Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATGCATTGGATGGATGCCCGGGCATTGAGAGATCGGAAGAGCACACGTC177210.21940508671645262No Hit
GGGAGAGTGACACCCAGGCAGACGTGCCCTCAGATCGGAAGAGCACACGT164420.2035696877033979No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC157190.19461816816748034TruSeq Adapter, Index 3 (100% over 50bp)
ACGTGTCGGTTCGAGTCCGACCCTCGGGACCAAGATCGGAAGAGCACACG154830.19169623371315592No Hit
CTGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACGT154100.19079241500482677No Hit
TCGATGGCCCGAGGTAGAGCACTCAATGGGCAGATCGGAAGAGCACACGT143740.17796561799347047No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCG131970.16339308895643728TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC122930.15220059426699128TruSeq Adapter, Index 3 (100% over 50bp)
ATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGA120430.1491053247179188Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGGGGTTCGAATCCCCGTAGGGACGCCAAGATCGGAAGAGCACACGTCT116210.14388050971908448Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGATGTCGTCCAGCGGTTAGGATATCTGGAGATCGGAAGAGCACACGT116070.14370717462433644No Hit
GCCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGAGATCGGAAGAGCACACG109900.13606804937722558No Hit
GACGTGTCGGTTCGAGTCCGACCCTCGGGACCAAGATCGGAAGAGCACAC103950.1287013078504331No Hit
GGGTACACGGAGTCGACCCGCAACGCGAAGATCGGAAGAGCACACGTCTG97590.12082694211759276Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTAATTGCTCGTGAGGCTTGACTAGATCGGAAGAGCACACGTCTGAACT96840.11989836125287101Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTG93310.11552784064958069Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGTACACGGAGTCGACCCGCAACGCGTAGATCGGAAGAGCACACGTCTG92560.11459925978485896Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTTCGAGCCCAGGTTGGAGAGCCAAGATCGGAAGAGCACACGTC90690.11228399816215276No Hit
CCGGGAAGAGCCCAGCTTGAAAATCGGACGAGATCGGAAGAGCACACGTC89660.1110087471079349No Hit
GGGTACACGGAGTCGACCCGCAACGCTGAGATCGGAAGAGCACACGTCTG86700.1073439479618331Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGGAGTTCGAATCTCTCTGAGCGCACCAAGATCGGAAGAGCACACGTC84530.10465725399323819No Hit
GATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAAC83360.10320866784427228Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGAGAGGTGATCCAGCCGCACCTTCCAGTACAGATCGGAAGAGCACACG82450.10208198972840991No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)