FastQCFastQC Report
Sun 2 Jul 2023
SRR3495995.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3495995.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8396815
Total Bases419.8 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG125754114.976404743941602TruSeq Adapter, Index 4 (100% over 49bp)
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGAGATCGGAAGAGCACACG3726374.437837441934829No Hit
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCTAGATCGGAAGAGCACAC1766572.1038572363449712No Hit
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCTAAGATCGGAAGAGCACA1313661.5644741488290501No Hit
CTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC1252891.4921014694262051No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAAGATCGGAAGAGCACACGT1046331.2461034332660657No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC650400.7745794089782853TruSeq Adapter, Index 4 (100% over 50bp)
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTAAGATCGGAAGAGCACAC526940.6275474688914785No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGTAGATCGGAAGAGCACAC511180.6087784475423121No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCG504290.6005729553408048TruSeq Adapter, Index 4 (100% over 50bp)
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTAGATCGGAAGAGCACACG469810.5595097665007506No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC416320.49580704112213975TruSeq Adapter, Index 4 (100% over 50bp)
TCCCAGTAAAACGCGGCGTGTTCGAATTAGATCGGAAGAGCACACGTCTG258230.30753327303269157Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCAGATCGGAAGAGCACACG246640.2937304204034506No Hit
ACCGGGGTTCGAATCCCCGTAGGGACGCCAAGATCGGAAGAGCACACGTC236850.28207123772525655No Hit
AACCGGGGTTCGAATCCCCGTAGGGACGCCAAGATCGGAAGAGCACACGT234990.27985611210917477No Hit
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAAC216090.25734757762318217Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGT185100.22044072663265774TruSeq Adapter, Index 4 (100% over 50bp)
TCGATGGCCCGAGGTAGAGCACTCAATGGGCAGATCGGAAGAGCACACGT179230.21344998073674362No Hit
ATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAACT170070.20254108254141598Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGTGTCGGTTCGAGTCCGACCCTCGGGACCAAGATCGGAAGAGCACACG164340.19571706653058332No Hit
TCCGTTGGTTCGAATCCAGCTACCCCTGCCAAGATCGGAAGAGCACACGT148870.17729341422908568No Hit
GGGAGAGTGACACCCAGGCAGACGTGCCCTCAGATCGGAAGAGCACACGT147250.17536411127314344No Hit
GAATGCATTGGATGGATGCCCGGGCATTGAGAGATCGGAAGAGCACACGT134420.16008450823318127No Hit
GTCTAGTGGTAGAATAGTACCCTAGATCGGAAGAGCACACGTCTGAACTC118910.14161321882166036Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTAATTGCTCGTGAGGCTTGACTAGATCGGAAGAGCACACGTCTGAACT114030.13580149139882206Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAACTC113320.1349559326959091Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGGGGTTCGAATCCCCGTAGGGACGCCAAGATCGGAAGAGCACACGTCT110080.13109732678402464Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGAGATCGGAAGAGCACACG109960.13095441545395486No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTCAGATCGGAAGAGCACACGTCT107080.1275245435322798Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCATTAAATCAGTTATAGTTTGTTTGATGAGATCGGAAGAGCACACGTC98810.11767557103496981No Hit
GACGTGTCGGTTCGAGTCCGACCCTCGGGACCAAGATCGGAAGAGCACAC97880.1165680082269289No Hit
ATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGA91390.1088388871256542Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTTCGAGCCCAGGTTGGAGAGCCAAGATCGGAAGAGCACACGTC84970.10119313096692019No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGAGATCGGAAGAGCACACGT84210.10028802587647816No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)