FastQCFastQC Report
Sun 2 Jul 2023
SRR3495996.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3495996.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12618680
Total Bases630.9 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG11676969.25370957976587TruSeq Adapter, Index 5 (100% over 49bp)
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGAGATCGGAAGAGCACACG6053804.7974907042574975No Hit
CTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC2168541.7185157243071383No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGTAGATCGGAAGAGCACAC922140.7307737417859872No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAAGATCGGAAGAGCACACGT706060.5595355457147657No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC408480.3237105624360076TruSeq Adapter, Index 5 (100% over 50bp)
AAGGTGCGTGCCGCACTCACGAGGGACTGCCAGAGATCGGAAGAGCACAC403930.3201047970152187No Hit
AAGGTGCGTGCCGCACTCACGAGGGACTGCCAAGATCGGAAGAGCACACG341950.2709871397008245No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGAGATCGGAAGAGCACACGT339920.269378413590011No Hit
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAAC334510.2650911188808972Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCAGTAAAACGCGGCGTGTTCGAATTCAGATCGGAAGAGCACACGTCT330310.26176272003093826Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC308020.24409843184865612TruSeq Adapter, Index 5 (100% over 50bp)
GAATGCATTGGATGGATGCCCGGGCATTGAGAGATCGGAAGAGCACACGT307510.2436942691311611No Hit
GGGAGAGTGACACCCAGGCAGACGTGCCCTCAGATCGGAAGAGCACACGT283580.22473032044556165No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCG237000.18781679224768358TruSeq Adapter, Index 5 (100% over 50bp)
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCTAGATCGGAAGAGCACAC229110.1815641572652607No Hit
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCTAAGATCGGAAGAGCACA214770.17020005262040086No Hit
AACCGGGGTTCGAATCCCCGTAGGGACGCCAAGATCGGAAGAGCACACGT191530.15178291231729468No Hit
ATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAACT186800.14803450123150758Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCGGGGTTCGAATCCCCGTAGGGACGCCAAGATCGGAAGAGCACACGTC184470.14618803234569702No Hit
GTCTAGTGGTAGAATAGTACCCTAGATCGGAAGAGCACACGTCTGAACTC161620.128079957650087Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGAGAGTCTCCGGACTCCCTAACGTTGCCGTCAGATCGGAAGAGCACAC146080.11576488190523888No Hit
CTGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACGT144540.11454446899358728No Hit
GGGAGAGGTGATCCAGCCGCACCTTCCAGTACAGATCGGAAGAGCACACG139140.11026509904364006No Hit
AAGGTGCGTGCCGCACTCACGAGGGACTGCCAGTAGATCGGAAGAGCACA137890.10927450414781893No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTCGGAGATCGGAAGAGCACACG133970.10616799855452393No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGCAGATCGGAAGAGCACACGT128890.10214222089790692No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)