FastQCFastQC Report
Sun 2 Jul 2023
SRR3495997.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3495997.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11542080
Total Bases577.1 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTGCGTGCCGCACTCACGAGGGACTGCCAAGATCGGAAGAGCACACGT5377094.658683703457262No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGAGATCGGAAGAGCACACG5296994.589285466744296No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG4259723.6905999611855056TruSeq Adapter, Index 6 (100% over 49bp)
CTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC1125940.9755087471235687No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGTAGATCGGAAGAGCACAC1091900.9460166625079708No Hit
GTCTAGTGGTAGAATAGTACCCTAGATCGGAAGAGCACACGTCTGAACTC725680.628725498350384Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCTAGATCGGAAGAGCACAC282400.2446699381740553No Hit
GAATGCATTGGATGGATGCCCGGGCATTGAGAGATCGGAAGAGCACACGT238830.20692110954004822No Hit
GGGAGAGTCTCCGGACTCCCTAACGTTGCCGTAGATCGGAAGAGCACACG228750.19818784829077601No Hit
GGGAGAGTGACACCCAGGCAGACGTGCCCAGATCGGAAGAGCACACGTCT228420.19790193795225816Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGAGAGTGACACCCAGGCAGACGTGCCCTCAGATCGGAAGAGCACACGT227020.19668898500097035No Hit
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCTAAGATCGGAAGAGCACA219350.19004373561784357No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTCAGATCGGAAGAGCACACGTCT206030.1785033546813053Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGGTGCGTGCCGCACTCACGAGGGACTGCCAAGATCGGAAGAGCACACG175110.1517144223571488No Hit
GGGAGAGTCTCCGGACTCCCTAACGTTGCCGTCAGATCGGAAGAGCACAC171470.14856074468380048No Hit
ATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAACT155840.13501899137763732Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACCGGGGTTCGAATCCCCGTAGGGACGCCAAGATCGGAAGAGCACACGT147060.12741204358313235No Hit
AAGGTGCGTGCCGCACTCACGAGGGACTGCCAGAGATCGGAAGAGCACAC145760.12628573012836508No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC143710.12450962044969366TruSeq Adapter, Index 6 (100% over 50bp)
GGGTACACGGAGTCGACCCGCAACGCGAAGATCGGAAGAGCACACGTCTG143700.12450095650004159Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGGGGTTCGAATCCCCGTAGGGACGCCAAGATCGGAAGAGCACACGTC142890.12379917657822506No Hit
TTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAACTC136070.11789036291552303Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC134920.11689400870553662TruSeq Adapter, Index 6 (100% over 50bp)
GGGAGAGGTGATCCAGCCGCACCTTCCAGTACAGATCGGAAGAGCACACG130040.11266600127533338No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGAGATCGGAAGAGCACACGT128790.11158300756882641No Hit
GCATCTCGGTTCGAGTCCGAGTAGCGGCACCAAGATCGGAAGAGCACACG126260.10939102830685629No Hit
TCCGTTGGTTCGAATCCAGCTACCCCTGCCAAGATCGGAAGAGCACACGT125250.10851596939199866No Hit
AATGCATTGGATGGATGCCCGGGCATTGAGAGATCGGAAGAGCACACGTC120620.10450456070309683No Hit
GGGTACACGGAGTCGACCCGCAACGCGTAGATCGGAAGAGCACACGTCTG119370.10342156699658987Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)