FastQCFastQC Report
Sun 2 Jul 2023
SRR3495998.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3495998.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11912468
Total Bases595.6 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG8546107.174080131841697TruSeq Adapter, Index 7 (100% over 49bp)
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGAGATCGGAAGAGCACACG3398392.852800947712934No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAAGATCGGAAGAGCACACGT2380991.9987377930417105No Hit
CTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC1327551.1144206221582293No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGTAGATCGGAAGAGCACAC495360.4158332261627062No Hit
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCTAAGATCGGAAGAGCACA494110.4147839054006273No Hit
GTCTAGTGGTAGAATAGTACCCTAGATCGGAAGAGCACACGTCTGAACTC457840.3843368141681472Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCTAGATCGGAAGAGCACAC338960.28454221241139954No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC281600.23639098128112496TruSeq Adapter, Index 7 (100% over 50bp)
AACCGGGGTTCGAATCCCCGTAGGGACGCCAAGATCGGAAGAGCACACGT271770.22813912280813683No Hit
GGGAGAGTGACACCCAGGCAGACGTGCCCTCAGATCGGAAGAGCACACGT267990.22496597682361033No Hit
ACCGGGGTTCGAATCCCCGTAGGGACGCCAAGATCGGAAGAGCACACGTC244390.20515480083556156No Hit
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAAC207310.1740277497492543Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC201460.16911692858272526TruSeq Adapter, Index 7 (100% over 50bp)
GAATGCATTGGATGGATGCCCGGGCATTGAGAGATCGGAAGAGCACACGT196140.16465101941931765No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCG190340.15978217108327175TruSeq Adapter, Index 7 (100% over 50bp)
TCCCAGTAAAACGCGGCGTGTTCGAATTCAGATCGGAAGAGCACACGTCT169170.1420108746567042Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAACT155810.13079573435160538Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGTTGGTTCGAATCCAGCTACCCCTGCCAAGATCGGAAGAGCACACGT146450.1229384204851589No Hit
ACGTGTCGGTTCGAGTCCGACCCTCGGGACCAAGATCGGAAGAGCACACG142710.11979885276501898No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTAGATCGGAAGAGCACACGTCTG134050.11252915852533664Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGAGTCTCCGGACTCCCTAACGTTGCCGTCAGATCGGAAGAGCACAC132050.11085024530601048No Hit
TTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAACTC131800.11064038115359472Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGAGACCGGGCTTGGACCATGTCTCCCGAACAATAGATCGGAAGAGCAC130390.10945674733396976No Hit
AATGCATTGGATGGATGCCCGGGCATTGAGAGATCGGAAGAGCACACGTC121410.10191842697919525No Hit
ATTCCCGTAGTAGCGGCGAGCGAAATGGGAGAGATCGGAAGAGCACACGT120910.10149869867436369No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)