FastQCFastQC Report
Sun 2 Jul 2023
SRR3495999.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3495999.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11442765
Total Bases572.1 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG6784735.9292749610780255TruSeq Adapter, Index 8 (100% over 49bp)
AGGTGCGTGCCGCACTCACGAGGGACTGCCAAGATCGGAAGAGCACACGT4234703.700766379454616No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGAGATCGGAAGAGCACACG3841713.3573266601210463No Hit
CTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC1297301.1337294788453665No Hit
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCTAAGATCGGAAGAGCACA1153561.0081129866776082No Hit
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCTAGATCGGAAGAGCACAC761550.6655297037036066No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGTAGATCGGAAGAGCACAC688290.6015067162525841No Hit
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAAC349540.3054681276771829Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC341760.2986690716797907TruSeq Adapter, Index 8 (100% over 50bp)
AGCATCTCGGTTCGAGTCCGAGTAGCGGCACCAAGATCGGAAGAGCACAC328380.28697609362772025No Hit
AACCGGGGTTCGAATCCCCGTAGGGACGCCAAGATCGGAAGAGCACACGT242680.21208160789809105No Hit
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTAGATCGGAAGAGCACACG222010.1940177920284127No Hit
GGGAGAGTGACACCCAGGCAGACGTGCCCTCAGATCGGAAGAGCACACGT217720.19026869816866815No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTAGATCGGAAGAGCACACGTCTG216740.18941226180909945Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGGGGTTCGAATCCCCGTAGGGACGCCAAGATCGGAAGAGCACACGTC216580.1892724354646801No Hit
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTAAGATCGGAAGAGCACAC205090.17923115610606352No Hit
ATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAACT193630.1692160941870256Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCAGTAAAACGCGGCGTGTTCGAATTCAGATCGGAAGAGCACACGTCT187000.16342204004014763Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC171160.14957923194262923TruSeq Adapter, Index 8 (100% over 50bp)
TCCGTTGGTTCGAATCCAGCTACCCCTGCCAAGATCGGAAGAGCACACGT161370.1410236074934686No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCG158280.13832321121686936TruSeq Adapter, Index 8 (100% over 50bp)
GAAGGGGTAGAGAAAATGCCCCGAGCCAATGTTCAGATCGGAAGAGCACA156100.13641807727415534No Hit
GTCTAGTGGTAGAATAGTACCCTAGATCGGAAGAGCACACGTCTGAACTC150770.1317601121756848Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGTGTCGGTTCGAGTCCGACCCTCGGGACCAAGATCGGAAGAGCACACG138000.12060022206171322No Hit
GGGAGAGTCTCCGGACTCCCTAACGTTGCCGTCAGATCGGAAGAGCACAC137630.12027687364024342No Hit
GGGAGACCGGGCTTGGACCATGTCTCCCGAACAATAGATCGGAAGAGCAC132970.11620443135902905No Hit
GCATCTCGGTTCGAGTCCGAGTAGCGGCACCAAGATCGGAAGAGCACACG126950.11094346515024996No Hit
GCTATACTCGACGAAGACTCCCCAGATCGGAAGAGCACACGTCTGAACTC120930.10568249894147089Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGAGAGTCTCCGGACTCCCTAACGTTGCCGTAGATCGGAAGAGCACACG119630.10454640989306344No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)