FastQCFastQC Report
Thu 6 Jul 2023
SRR3498206.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3498206.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163396264
Total Bases8.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC1648354710.088080716459956No Hit
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCTAGATCGGAAGAGCACAC45622342.7921287110946427No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAAGATCGGAAGAGCACACGT40865622.501013119859338No Hit
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTAGATCGGAAGAGCACACG32473531.9874095774919311No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG27510871.6836902709109678TruSeq Adapter, Index 4 (100% over 49bp)
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCTAAGATCGGAAGAGCACA27216471.6656727230923714No Hit
CTGATGATTCATGATAACTCGACGGAGATCGGAAGAGCACACGTCTGAAC23092921.4133077118580875Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGAGATCGGAAGAGCACACG22786021.3945251526681173No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTAGATCGGAAGAGCACACGTCTG20070651.2283420384691293Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTAAGATCGGAAGAGCACAC17747051.0861356046671913No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGAGATCGGAAGAGCACACG13464240.8240237365525076No Hit
CTGATGATTCATGATAACTCGACGAGATCGGAAGAGCACACGTCTGAACT9879360.6046258193516592Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGA8821230.539867300760316Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCT6744250.41275423531103506Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGTAGATCGGAAGAGCACAC6008100.36770118562808757No Hit
AAGCCAGTGGCGCGAAGCTACCGTGCGCTGGAGATCGGAAGAGCACACGT5670310.34702813033717833No Hit
AAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGAGATCGGAAGAGCACAC4821930.2951065025574881No Hit
ATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAACT4372500.26760097770656494Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCAGATCGGAAGAGCACACG4342440.26576127836068514No Hit
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAAC4207660.25751261974998396Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGAATCCTTTGCAGACGACTTAAATAAGATCGGAAGAGCACACGTCTGA3647360.22322175003952355Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGATGGCCCGAGGTAGAGCACTCAATGGGCAGATCGGAAGAGCACACGT3609860.22092671592540206No Hit
TTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAACTC3561770.21798356417745268Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACGT3489800.21357893470563072No Hit
GATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTG3187470.1950760636730348Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGAGAGGGTGAGAGCCCCGTCGTGCCCGGAGATCGGAAGAGCACACGTC3113010.19051904393603517No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGAGATCGGAAGAGCACACGT2614070.15998346204537456No Hit
CGGCTCAGCCGGAGGTAGGGTCCAGCGGCTGGAAGAGATCGGAAGAGCAC2552030.15618655760697198No Hit
CCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGAGATCGGAAGAGCACACGT2537240.15528139615236244No Hit
AGAGAGGGTGAGAGCCCCGTCGTGCCCGGAGATCGGAAGAGCACACGTCT2533630.15506046086830969Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGAGGGCACGTCTGCCTGGGTGTCACAAGATCGGAAGAGCACACGTCTGA2523560.15444416770753094Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTATTAGACTGATGATTCATGATAACTCAGATCGGAAGAGCACACGTCTG2510990.15367487227247742Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAGCCAGTGGCGCGAAGCTACCGTGCGCTGAGATCGGAAGAGCACACGT2500850.15305429504801898No Hit
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCAGATCGGAAGAGCACACG2339220.14316239201160683No Hit
CGGCTCAGCCGGAGGTAGGGTCCAGCGGCTGGAGATCGGAAGAGCACACG2288150.1400368615527219No Hit
AGCCAGTGGCGCGAAGCTACCGTGCGCTGGAGATCGGAAGAGCACACGTC2260410.13833914831736913No Hit
CTGATGATTCATGATAAATCGACGGATCGCAGATCGGAAGAGCACACGTC2118450.12965106717495084No Hit
GCCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGAGATCGGAAGAGCACACG2116630.1295396815192788No Hit
CTGATGATTCATGATAACTCGACGGATCGAGATCGGAAGAGCACACGTCT1989200.12174084959494545Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCGCGACCTATACCCGGCCGTCGGGGCAGATCGGAAGAGCACACGTC1976570.12096788210530934No Hit
TGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAA1968550.12047705080943588Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAATGCATTGGATGGATGCCCGGGCATTGAGAAGATCGGAAGAGCACACG1903520.11649715565100068No Hit
CGGATAACCGTAGTAATTCTAGAGCTAATACGTAGATCGGAAGAGCACAC1887740.11553140529577838No Hit
TTATTAGCTGATGATTCATGATAACTCAGATCGGAAGAGCACACGTCTGA1871900.11456198288597345Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGGATACTCGGCTCTCACCCGAGAGACCCGGGAGATCGGAAGAGCACAC1841280.11268801103065612No Hit
AAAGCCCGATCCAGCAATATCGCGTGAGTGAAAGATCGGAAGAGCACACG1829030.11193829988670977No Hit
GAATGCATTGGATGGATGCCCGGGCATTGAGAGATCGGAAGAGCACACGT1816360.11116288436068528No Hit
TCTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGT1741390.10657465215973358No Hit
TCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAACTCC1720490.10529555314679655Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTAAGTCAGAATCCGGGCTAGAAGCGACGCATAGATCGGAAGAGCACACG1684720.10310639660647322No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)