FastQCFastQC Report
Mon 10 Jul 2023
SRR3498207.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3498207.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166148265
Total Bases8.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC96389435.80141056543684No Hit
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCTAGATCGGAAGAGCACAC60337143.6315239283419545No Hit
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCTAAGATCGGAAGAGCACA56357163.3919800486631626No Hit
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTAGATCGGAAGAGCACACG30731891.849666621556355No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG30660561.8453734680888783TruSeq Adapter, Index 5 (100% over 49bp)
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTAAGATCGGAAGAGCACAC21038791.2662660064491194No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTAGATCGGAAGAGCACACGTCTG19143901.15221786998498Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGTGCGTGCCGCACTCACGAGGGACTGCCAAGATCGGAAGAGCACACGT11792840.7097781009028292No Hit
CTGATGATTCATGATAACTCGACGGAGATCGGAAGAGCACACGTCTGAAC10706650.6444033586507809Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGAGATCGGAAGAGCACACG8542320.5141383811621505No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGAGATCGGAAGAGCACACG7134230.4293893770121524No Hit
CGAGGGCACGTCTGCCTGGGTGTCACAAGATCGGAAGAGCACACGTCTGA5258180.316475167525824Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGATGATTCATGATAACTCGACGAGATCGGAAGAGCACACGTCTGAACT4395150.26453180236338913Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGA3888100.23401387910972166Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGATGGCCCGAGGTAGAGCACTCAATGGGCAGATCGGAAGAGCACACGT3667510.22073718314181615No Hit
TGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCT3614080.21752138067767365Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGCTCATTAAATCAGTTATAGTTTGTTTGATGAGATCGGAAGAGCACA3444860.20733650152771682No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGAGATCGGAAGAGCACACGT3228220.19429754502702754No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGTAGATCGGAAGAGCACAC3063180.18436424840187168No Hit
AACCGGGGTTCGAATCCCCGTAGGGACGCCAAGATCGGAAGAGCACACGT2931470.17643699138236563No Hit
TGGTACACGGAGTCAAGACCCGCAACGCGTAGATCGGAAGAGCACACGTC2793080.16810768382083316No Hit
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCAGATCGGAAGAGCACACG2636180.1586643110597634No Hit
CAGGATACTCGGCTCTCACCCGAGAGACCCGGGAGATCGGAAGAGCACAC2519710.15165430707326377No Hit
ACCGGGGTTCGAATCCCCGTAGGGACGCCAAGATCGGAAGAGCACACGTC2473100.14884898136011232No Hit
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAAC2442600.14701327154996172Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGAATCCTTTGCAGACGACTTAAATAAGATCGGAAGAGCACACGTCTGA2404340.1447105090143433Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACACGGAGTCAAGACCCGCAACGCGTAGATCGGAAGAGCACACGTCTGAA2335740.14058166662167673Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGTACACGGAGTCAAGACCCGCAACGCGGAGATCGGAAGAGCACACGTC2309440.13899874308046492No Hit
AGAGAGGGTGAGAGCCCCGTCGTGCCCGGAGATCGGAAGAGCACACGTCT2271270.1367013973934666Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCAGATCGGAAGAGCACACG2162880.13017770603863965No Hit
GAATGCATTGGATGGATGCCCGGGCATTGAGAGATCGGAAGAGCACACGT2147920.1292773054235625No Hit
CTCATTAAATCAGTTATAGTTTGTTTGATGAGATCGGAAGAGCACACGTC2142570.12895530386670004No Hit
CAGGATACTCGGCTCTCACCCGAGAGACCCGGGTAGATCGGAAGAGCACA2120080.1276016935837398No Hit
CTGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACGT2079630.1251671210650319No Hit
AAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGAGATCGGAAGAGCACAC2001100.12044061970794578No Hit
ATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAACT1988280.11966901971561365Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGTACACGGAGTCAAGACCCGCAACGCGGAGATCGGAAGAGCACACGTC1948590.11728018947414226No Hit
GGGTACACGGAGTCAAGACCCGCAACGCGTAGATCGGAAGAGCACACGTC1871590.11264577454359816No Hit
TGGCTCATTAAATCAGTTATAGTTTGTTTGATGAGATCGGAAGAGCACAC1725950.10388010973211186No Hit
TGGTACACGGAGTCGACCCGCAACGCGTAGATCGGAAGAGCACACGTCTG1718050.1034046307976794Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGTACACGGAGTCAAGACCCGCAACGCGGAGATCGGAAGAGCACACGTC1710650.10295924546669206No Hit
TTATTAGCTGATGATTCATGATAACTCAGATCGGAAGAGCACACGTCTGA1683630.1013329871365193Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGAGAGGGTGAGAGCCCCGTCGTGCCCGGAGATCGGAAGAGCACACGTC1678570.10102843986965497No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)