FastQCFastQC Report
Thu 13 Jul 2023
SRR3498208.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3498208.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207346547
Total Bases10.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC172475708.318233532000898No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG93056364.487962849943192TruSeq Adapter, Index 6 (100% over 49bp)
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCTAGATCGGAAGAGCACAC43485452.0972353110852624No Hit
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCTAAGATCGGAAGAGCACA40194261.9385063595971048No Hit
ATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAACT20878841.0069538317414082Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTAGATCGGAAGAGCACACG20703830.9985133728800413No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAAGATCGGAAGAGCACACGT20447660.9861586940244537No Hit
CTGATGATTCATGATAACTCGACGGAGATCGGAAGAGCACACGTCTGAAC18476640.89109947897999Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCAGTAAAACGCGGCGTGTTCGAATTAGATCGGAAGAGCACACGTCTG17383710.8383891726926129Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGAGATCGGAAGAGCACACG13913590.6710307068677638No Hit
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTAAGATCGGAAGAGCACAC13670060.6592856354632228No Hit
AAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGAGATCGGAAGAGCACACG13106920.6321262731228411No Hit
TTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAACTC12737720.6143203339672688Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGA9892040.4770776337066274Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGATGATTCATGATAACTCGACGAGATCGGAAGAGCACACGTCTGAACT8373400.40383599925587377Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAACTCC7501370.3617793548305388Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCT7367190.35530806307567786Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGTAGATCGGAAGAGCACAC5794660.2794673981235868No Hit
TAGAATCCTTTGCAGACGACTTAAATAAGATCGGAAGAGCACACGTCTGA5282070.2547459833030159Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACGT4235590.2042758879413603No Hit
CTGATGATTCATGATAACTCGACGTATCGCAGATCGGAAGAGCACACGTC3567920.17207520702044776No Hit
TGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAA3557420.17156880842582828Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTG3314150.15983627641505888Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCCAGTGGCGCGAAGCTACCGTGCGCTGGAGATCGGAAGAGCACACGT3300690.1591871216451943No Hit
TCGATGGCCCGAGGTAGAGCACTCAATGGGCAGATCGGAAGAGCACACGT3288930.1586199552192205No Hit
AAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGAGATCGGAAGAGCACAC3108360.1499113462448931No Hit
CTCCCGGCAGACGCACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC3069400.14803236631666694Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGAGGGCACGTCTGCCTGGGTGTCACAAGATCGGAAGAGCACACGTCTGA3013110.14531758756513077Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGAGAGGGTGAGAGCCCCGTCGTGCCCGGAGATCGGAAGAGCACACGTC2768560.13352332315425536No Hit
TTATTAGACTGATGATTCATGATAACTCAGATCGGAAGAGCACACGTCTG2666270.12859003627391008Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGATGATTCATGATAACTCGACGGATCGAGATCGGAAGAGCACACGTCT2662230.12839519338607555Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCAGATCGGAAGAGCACACG2483820.11979075783692698No Hit
GATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAAC2279660.10994444002002117Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TACCTGGTTGATCCTGCCAGTAGTCATATAGATCGGAAGAGCACACGTCT2261830.10908452697791973Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCATTAAATCAGTTATAGTTTGTTTGATGAGATCGGAAGAGCACACGTC2203430.10626798622308381No Hit
CCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGAGATCGGAAGAGCACACGT2184080.10533476595585649No Hit
AAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATC2164420.10438659487297852TruSeq Adapter, Index 6 (100% over 42bp)
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCAGATCGGAAGAGCACACG2100400.10129901029892724No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)