FastQCFastQC Report
Mon 10 Jul 2023
SRR3498210.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3498210.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179566257
Total Bases8.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC123864536.8979847366312255No Hit
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCTAAGATCGGAAGAGCACA40524452.256796498241872No Hit
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCTAGATCGGAAGAGCACAC24015831.3374355739898283No Hit
CTGATGATTCATGATAACTCGACGGAGATCGGAAGAGCACACGTCTGAAC20383581.1351564787587012Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGAGATCGGAAGAGCACACG15286830.8513197443325892No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAAGATCGGAAGAGCACACGT13711740.7636033756609406No Hit
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTAAGATCGGAAGAGCACAC9237390.5144279417708194No Hit
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTAGATCGGAAGAGCACACG8369290.46608366960614434No Hit
CTGATGATTCATGATAACTCGACGAGATCGGAAGAGCACACGTCTGAACT7951100.44279477296227204Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGTAGATCGGAAGAGCACAC7895980.43972515393022865No Hit
AGAGAGGGTGAGAGCCCCGTCGTGCCCGGACCCAGATCGGAAGAGCACAC6969600.3881352831228197No Hit
AGAGAGGGTGAGAGCCCCGTCGTGCCCGGAAGATCGGAAGAGCACACGTC6636510.36958558422254134No Hit
AGCATCTCGGTTCGAGTCCGAGTAGCGGCACCAAGATCGGAAGAGCACAC6560100.36533033040834617No Hit
ATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGA6390060.3558608452811933Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGGATACTCGGCTCTCACCCGAGAGACCCGGGAGATCGGAAGAGCACAC5942500.33093634067340394No Hit
AGAGAGGGTGAGAGCCCCGTCGTGCCCGGACCAGATCGGAAGAGCACACG5803570.32319936367554847No Hit
CAGAGAGGGTGAGAGCCCCGTCGTGCCCGGAGATCGGAAGAGCACACGTC5603940.3120820188394304No Hit
TGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCT5242110.2919317965178725Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCAGTAAAACGCGGCGTGTTCGAATTAGATCGGAAGAGCACACGTCTG5122400.2852651765192165Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGAAAAGCCCAAAGCCAATCCCAGGGAACAGATCGGAAGAGCACACGTC4910890.27348623745050277No Hit
ATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAACT4806920.26769617412028585Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACCGGGCCTAAGTTCCCTGGAAAGGGGCGCCAGATCGGAAGAGCACACG4777720.26607003341390584No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG4689660.2611659940096652TruSeq Adapter, Index 8 (100% over 49bp)
CAGGATACTCGGCTCTCACCCGAGAGACCCGGGTAGATCGGAAGAGCACA4054450.22579130777337525No Hit
AGAGAGGGTGAGAGCCCCGTCGTGCCCGGAGATCGGAAGAGCACACGTCT3716670.20698042394457217Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGAGAGTCTCCGGACTCCCTAACGTTGCCGTCAGATCGGAAGAGCACAC3399790.18933345589533562No Hit
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAAC3392030.18890130343364012Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAAGGGGTAGAGAAAATGCCCCGAGCCAATGTTCAGATCGGAAGAGCACA3337080.1858411516591338No Hit
AGAAAAGATAACTCTTCCCGAGGCCCCCGCAGATCGGAAGAGCACACGTC2891970.16105308693937972No Hit
CTGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACGT2878210.16028679597637321No Hit
GGACCGGGCCTAAGTTCCCTGGAAAGGGGCGCAGATCGGAAGAGCACACG2673120.1488653851040622No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGAGATCGGAAGAGCACACGT2511210.13984865764618573No Hit
ATTCCCGTAGTAGCGGCGAGCGAAATGGGAGAGATCGGAAGAGCACACGT2383200.13271981272071623No Hit
TTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAACTC2372780.13213952552343952Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGACCGGGCCTAAGTTCCCTGGAAAGGGGCGCCAGATCGGAAGAGCACAC2293740.1277378076661697No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTCAGATCGGAAGAGCACACGTCT2274390.12666021099944186Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTG2202590.12266168693375393Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACCGGGCCTAAGTTCCCTGGAAAGGGGCGCAGATCGGAAGAGCACACGT2158530.12020799653912706No Hit
TCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAGATCGGAAGAGCA2105710.11726646393258616No Hit
AACCGGGGTTCGAATCCCCGTAGGGACGCCAAGATCGGAAGAGCACACGT2061120.11478325797034351No Hit
ACCGGGCCTAAGTTCCCTGGAAAGGGGCGCCAGATCGGAAGAGCACACGT2035150.1133369951571692No Hit
ACCGGGGTTCGAATCCCCGTAGGGACGCCAAGATCGGAAGAGCACACGTC1806100.10058125786962303No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)