FastQCFastQC Report
Mon 3 Jul 2023
SRR3498211.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3498211.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134241510
Total Bases6.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC1664518712.399433677407234No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG33854022.521874195247059TruSeq Adapter, Index 3 (100% over 49bp)
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCTAAGATCGGAAGAGCACA30163332.2469450768245975No Hit
CTGATGATTCATGATAACTCGACGGAGATCGGAAGAGCACACGTCTGAAC24006371.7882970774092157Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCTAGATCGGAAGAGCACAC18219371.3572083627486013No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGAGATCGGAAGAGCACACG13964721.0402683938820414No Hit
CTGATGATTCATGATAACTCGACGAGATCGGAAGAGCACACGTCTGAACT10299170.7672120195906617Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGTGCGTGCCGCACTCACGAGGGACTGCCAAGATCGGAAGAGCACACGT8950280.6667296874118892No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGTAGATCGGAAGAGCACAC8168220.6084719994582898No Hit
ATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGA7415000.552362678280362Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCT6533220.48667658759201976Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTAAGATCGGAAGAGCACAC6312850.4702606518654327No Hit
AGCATCTCGGTTCGAGTCCGAGTAGCGGCACCAAGATCGGAAGAGCACAC6235810.46452174144942204No Hit
ATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAACT5629420.41935016970533184Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAGGATACTCGGCTCTCACCCGAGAGACCCGGGAGATCGGAAGAGCACAC5333720.39732270592009883No Hit
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTAGATCGGAAGAGCACACG5313590.395823169748314No Hit
CTGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACGT3757160.2798806419862232No Hit
GAAGGGGTAGAGAAAATGCCCCGAGCCAATGTTCAGATCGGAAGAGCACA3693080.2751071557523452No Hit
CGGCTCAGCCGGAGGTAGGGTCCAGCGGCTGGAGATCGGAAGAGCACACG3659630.2726153780600352No Hit
GATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTG3460230.2577615522948155Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAACTC2898550.21592054499386965Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTCCCGTAGTAGCGGCGAGCGAAATGGGAGAGATCGGAAGAGCACACGT2873660.21406642401444975No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTAGATCGGAAGAGCACACGTCTG2869200.21373418698880844Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAAC2695450.20079109658405958Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAGGATACTCGGCTCTCACCCGAGAGACCCGGGTAGATCGGAAGAGCACA2389150.17797401116837855No Hit
CAGAGAGGGTGAGAGCCCCGTCGTGCCCGGAGATCGGAAGAGCACACGTC2184950.16276262089125784No Hit
CTGATGATTCATGATAACTCGACGGATCGAGATCGGAAGAGCACACGTCT2170420.16168024331669092Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAA1869060.13923115137784134Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACCGGGGTTCGAATCCCCGTAGGGACGCCAAGATCGGAAGAGCACACGT1633690.12169782655156366No Hit
GCCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGAGATCGGAAGAGCACACG1536570.11446310459410058No Hit
CTGATGATTCATGATAACTCGACGGATAGATCGGAAGAGCACACGTCTGA1495340.11139177442208449Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAAC1462970.10898044874495227Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGGGGTTCGAATCCCCGTAGGGACGCCAAGATCGGAAGAGCACACGTC1378750.10270668141322307No Hit
CCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGAGATCGGAAGAGCACACGT1366600.10180159624247373No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)