FastQCFastQC Report
Sun 2 Jul 2023
SRR3590722.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590722.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11414723
Total Bases570.7 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1395861.222859284452194No Hit
ACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA858660.7522390162249228Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA814890.7138938018907687Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTGAGCGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC551090.48278876324900744No Hit
ATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT550620.4823770143173864No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA457940.40118362924794587No Hit
AATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG364420.31925435247092726Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG335200.29365583378589216No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC315210.27614336326864874No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC280720.24592800018011823No Hit
TTTTGAGCGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT275020.24093444930726746No Hit
ACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT251550.22037328457291516No Hit
CGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT240720.21088553791449868Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG220190.19289999415666942Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGAGCGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT188990.16556687358948613Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC170770.14960503202749642No Hit
TGGGAGAGGTCTCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC164560.14416468976075897No Hit
TGGGAGAGGTCTCCGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA143500.12571483337791026No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)