FastQCFastQC Report
Sun 2 Jul 2023
SRR3590723.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590723.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17675936
Total Bases883.7 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA14286678.082553591504292No Hit
TAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAG8222564.651838522158035No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA6828103.8629354620881178No Hit
AGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGA5084562.87654356748067No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA3577702.0240512298754645No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG3084811.7452031960287704No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA2631651.4888320482717294No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA2503101.4161060551475182No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG1987601.124466619476332No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA1807291.022457877195301No Hit
ATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGC1492360.8442890945067916No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG1390150.7864647167765261No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1368100.7739901298579039No Hit
TTAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAA1177360.6660807099550484No Hit
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG1078300.6100384160703003No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGATCG1050760.594457911592348No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA879500.49756912448653356No Hit
TTGTGGCGTCGCTGAACCATAGCTGTAGGCACCATCAATAGATCGGAAGA677250.3831480267862477No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT660850.3738698759714903No Hit
ATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAG659630.3731796720694169No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT628830.35575485224657977No Hit
TTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATCGG624730.3534353145428904No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGA624430.35326559227188875No Hit
TTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATCG604550.3420186631135121No Hit
TTCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA598270.33846581024054395No Hit
ATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAA595270.33676858753052735No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT558750.316107729740592No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA533400.30176619784095166No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAG521370.2949603347737851No Hit
TCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAG519340.2938118807400072No Hit
GAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAA513690.2906154446361426No Hit
TTATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG505230.2858292765938958No Hit
TGTGGCGTCGCTGAACCATAGCTGTAGGCACCATCAATAGATCGGAAGAG465140.2631487237790406No Hit
ATAAATCATTTGTATACGACTTAGATCTGTAGGCACCATCAATAGATCGG453830.25675019416227807No Hit
TGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGC446590.25265423002210463No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG445810.2522129521175003No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGA440860.24941253464597293No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT411190.23262700204390876No Hit
ATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGC405930.2296512048923463No Hit
ATAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA391520.22149887847523322No Hit
AAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGAAGA376120.21278646856381467No Hit
ATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAG359300.20327070656965493No Hit
ATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGAGC356220.2015282245873712No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG354960.20081539104916424No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT351790.19902199238558002No Hit
ATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGATC346980.19630077864052006No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG345100.19523718574224302No Hit
TGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGA336610.19043404547289602No Hit
TAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAA334030.18897443394228175No Hit
AGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAG328460.18582325711068426No Hit
TCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATCGGAA322060.18220251532931553No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA318970.18045437593799843No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA313440.17732582874253447No Hit
TTGTGGCGTCGCTGAACCATAGCCTGTAGGCACCATCAATAGATCGGAAG309510.17510246699241275No Hit
CATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA300110.1697845025010274No Hit
TTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAG284900.16117958336124322No Hit
AGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGAA265840.1503965617436044No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG264850.14983647824929894No Hit
TTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAA260910.1476074590901438No Hit
ATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGA260890.147596144272077No Hit
ATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGA260820.1475565424088433No Hit
TCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAGA260230.14722275527587336No Hit
ATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGA250740.1418538741031875No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCACCATCAATAGA246550.13948341971819767No Hit
AATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA241790.13679049301830465No Hit
CACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAG240600.1361172613433314No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG239140.13529127962445667No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATC236680.13389955700224304No Hit
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA235310.1331244919646688No Hit
TTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATCGGA231430.130929417259714No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGAT222710.12599615658259908No Hit
ACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATCGGAAGAGCA220030.12447997096165092No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATC217800.12321836874720524No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT216890.12270354452516687No Hit
TATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGA216320.12238107221026372No Hit
ATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAGATCGGAAGAG211990.11993141409880642No Hit
TTGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGATC210270.11895833974506356No Hit
TTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGA209540.1185453488856262No Hit
ATTTGTATACGACTTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCA207230.11723848739891342No Hit
TGTGGCGTCGCTGAACCATAGCCTGTAGGCACCATCAATAGATCGGAAGA205830.11644645013423899No Hit
ACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAGAGC198140.11209590258756312No Hit
GCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA197850.11193183772559485No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG195120.11038736505947974No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGG191840.10853173489652826No Hit
ACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAGAG187460.10605378973990401No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGATC187180.10589538228696913No Hit
GTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAG181910.10291392772637331No Hit
AGATGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA179280.10142602915059208No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)