FastQCFastQC Report
Sun 2 Jul 2023
SRR3590724.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590724.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19983038
Total Bases999.1 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA577380.28893504581235346Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC414050.20720072693651484No Hit
ACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT362190.181248717036919No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA274720.137476593899286No Hit
AATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG219920.11005333623446045No Hit
AATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG206070.10312245815676274Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)