FastQCFastQC Report
Sun 2 Jul 2023
SRR3590725.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590725.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38468915
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1710020.44451994551964874Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1346960.3501424461802471No Hit
ACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT966690.2512912048598199No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA846450.22003479952579894No Hit
AATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG688300.17892368422660218No Hit
TCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT664020.17261209472635244No Hit
ATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT587020.15259593362588988No Hit
AATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG586230.15239057301200204Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC556730.14472204375922743No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC547650.1423616964502378No Hit
AATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC533870.13877958346368752No Hit
TTTGAGCGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC521890.1356653807366285No Hit
ATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG480970.12502822083752557No Hit
TCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA474870.12344252495813829No Hit
GCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA467370.12149289887692438No Hit
TTTTGAGCGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT416730.10832902357656826No Hit
ACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG387400.10070468584830117Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)