FastQCFastQC Report
Sun 2 Jul 2023
SRR3590726.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590726.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10157184
Total Bases507.8 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA630360.6206050810933424No Hit
TCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC616040.6065066853175053No Hit
AATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG468380.46113174675185564No Hit
ATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT452370.44536950398850705No Hit
ACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT444230.43735547175280076No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC370610.364874752687359No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA323610.3186020849873351Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC283410.2790241862311444No Hit
CAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC262250.2581916405176868No Hit
TACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCAC182160.17934104570715662No Hit
CACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCAC167500.16490791148412787No Hit
TGGGAGAGGTCTCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC123500.12158881831814802No Hit
TTTTGAGCGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT122450.12055506723123259No Hit
TTTGAGCGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC112820.1110740929769511No Hit
AATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG110410.108701388101269Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)