FastQCFastQC Report
Sun 2 Jul 2023
SRR3590728.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590728.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9984949
Total Bases499.2 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4797124.804351028733347Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA267300.26770291966438686No Hit
ACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT124980.12516839094521165No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC107360.10752183110800065No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)