FastQCFastQC Report
Sun 2 Jul 2023
SRR3590729.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590729.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19178497
Total Bases958.9 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA12289536.407973471539506Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA781530.40750325742418714No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA628990.32796626346684Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC337080.17575934130813273No Hit
ACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT315830.1646792238203025No Hit
AATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC299900.1563730463341314No Hit
AATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG267350.13940091342924318No Hit
ACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCAC237170.12366453951005649No Hit
ATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG222710.11612484544539649No Hit
TGGGAGAGGTCTCCGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA197440.10294863043751551No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)