FastQCFastQC Report
Sun 2 Jul 2023
SRR3590730.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590730.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12268615
Total Bases613.4 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA481850.39275011890095174No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC370480.30197377617603943No Hit
TCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC356770.29079892066056356No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC337760.2753040991179526No Hit
ATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT314420.2562799468399652No Hit
AATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG272820.22237228896660302No Hit
ACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT236520.19278459712037585No Hit
CAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC170860.1392659236596796No Hit
TCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT168280.13716299680118743No Hit
TGGGAGAGGTCTCCGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA160550.13086236710500737No Hit
TGGGAGAGGTCTCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC154750.12613485711304823No Hit
AATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC152020.12390966706510881No Hit
ATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG149440.12180674020661664No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)