FastQCFastQC Report
Sun 2 Jul 2023
SRR3590731.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590731.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1172094
Total Bases58.6 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA51954644.32630829950499Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC232111.9803019211769703Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA220441.8807365279576553No Hit
TAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAG91750.78278704609016No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA73800.6296423324409135No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC70580.6021701331121907Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA65730.5607911993406672No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA58830.5019222007791184No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG56620.4830670577615788No Hit
AGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGA55500.47351151016897963No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA48270.4118270377631828No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA47290.4034659336196585Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA36730.3133707706037229No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA32470.277025562796158No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG29500.2516862981979261No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA29230.24938272868899594No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC27950.2384621028688825No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA27720.2364998029168309No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC24860.21209902960001503No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG24720.2109045861509401No Hit
TCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC22830.19477959958842891No Hit
CTGTAGGCACCATCAATAGATAGGAAGAGCACACGTCTGAACTCCAGTCA21890.18675976500178312Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGGCACCATCAATAGATCGAAGAGCACACGTCTGAACTCCAGTCAC20640.17609509134932863Illumina Multiplexing Index Sequencing Primer (96% over 29bp)
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC19990.1705494610500523Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGC18830.16065264390057452No Hit
ACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT18520.1580078048347658No Hit
GATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACA18020.15374193537378403No Hit
CAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC17810.15195027020017166No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG17740.1513530484756342No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT17360.14811098768528805No Hit
ATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC17090.14580741817635787No Hit
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG16550.1412002791584975No Hit
CTGTAGGCACCATCAATAATCGGAAGAGCACACGTCTGAACTCCAGTCAC16520.14094432699083861Illumina Multiplexing Index Sequencing Primer (96% over 33bp)
TATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACA15830.13505742713468374No Hit
AATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG15580.13292449240419282No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT15430.1316447315658983No Hit
AATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG14390.12277172308705615Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGAGAGGTCTCCGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA14340.12234513614095797No Hit
ATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT14160.12080942313500453No Hit
CTGTAGGCACCATAAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA13790.11765267973387801Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGC13700.11688482323090128No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT13490.11509315805728892No Hit
TTAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAA13240.11296022332679802No Hit
TCCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA12890.10997411470411075No Hit
CTGTAGGAACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA12800.10920625820113404Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAA11740.10016261494385263No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)