FastQCFastQC Report
Sun 2 Jul 2023
SRR3590732.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590732.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1497641
Total Bases74.8 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA66185644.19323456021837Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGGCTGAACTCCAGTCA141370.9439511872337898Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGGCA118120.7887070399381426Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC92990.6209098175063316Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC66960.44710314421146324Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGTAGGCACCATCAATAGATCGGAAGATCACACGTCTGAACTCCAGTCA62910.42006061532770533Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGGCACCATCAATAGATCGGAATAGCACACGTCTGAACTCCAGTCA49040.3274483003603667Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGGCACCATCAATAGAGCGGAAGAGCACACGTCTGAACTCCAGTCA47090.31442782349040926Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACGCCAGTCA46930.31335947667031017Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGGCACCATCAATAGATCGTAAGAGCACACGTCTGAACTCCAGTCA45330.3026760084693194Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTATGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA44770.29893679459897265Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGTAGGCACCATCACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA44050.2941292339085268Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA43650.29145836685827914Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACACTTCTGAACTCCAGTCA43250.2887874998080314Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGTCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA41900.27977332351344547Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGTAGGCACCATCAATAGATCGGCAGAGCACACGTCTGAACTCCAGTCA39140.2613443408667364Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTACAGTCA35370.23617141891815194Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTGCAGTCA33820.22582180909844216Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGGCACCATCAATAGATCTGAAGAGCACACGTCTGAACTCCAGTCA33450.22335125707696304Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCGGAACTCCAGTCA32610.2177424362714429Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGGCACCATCAATAGATAGGAAGAGCACACGTCTGAACTCCAGTCA31880.21286810390474084Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGGCACCATCAATATATCGGAAGAGCACACGTCTGAACTCCAGTCA31300.2089953466818817Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTTAACTCCAGTCA29710.19837865015714715Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGGCACCATCAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA29200.19497329466808133Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTCAACTCCAGTCA29110.1943723495817756Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGGAACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA28740.19190179756029652Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCGAGTCA27770.18542494496344583Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGGCACCATAAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA27530.18382242473329724Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGCACTCCAGTCA27140.18121832935930574Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCATTCA26970.18008321086295048Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGGCACCAGCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA26750.17861423398531423Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGTAGGCACCATCAATAGATCGGAAGCGCACACGTCTGAACTCCAGTCA26130.17447439005743032Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA25930.17313895653230646No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCCGTCA25460.17000068774826543Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA24240.16185454324501No Hit
CTGTAGGCACCATCAATAGCTCGGAAGAGCACACGTCTGAACTCCAGTCA23760.15864950278471276Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGGCACCATCAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA22250.14856697967002774Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGACA21840.14582934094352384Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGGCACCATCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA20870.1393524883466732Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC20780.13875154326036745Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGCCA20530.13708225135396268Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
ACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT19790.13214114731100443No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCAAGTCA19710.1316069739009549Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGCCTGAACTCCAGTCA19240.12846870511691386Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
AATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG18720.12499657795159186No Hit
CTGTAGGCACAATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA18680.12472949124656711Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC18620.12432886118902994No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG17500.11685043344833641No Hit
CTGTAGGCACCATCAATAGATCGGAACAGCACACGTCTGAACTCCAGTCA16900.11284413287296488Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAGCTCCAGTCA16780.11204287275789057Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACA16720.11164224270035343No Hit
CTGTAGGCACCCTCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA16320.10897137565010573Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGTAAGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA16150.10783625715375046Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC16030.10703499703867615No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG15740.10509861842724659No Hit
CTGTAGGCAACATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA15440.1030954681395608Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC15360.10256129472951128No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGACCTCCAGTCA15200.1014929479094122Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGGACTCCAGTCA15170.10129263288064362Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)