FastQCFastQC Report
Sun 2 Jul 2023
SRR3590733.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590733.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2904454
Total Bases145.2 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA187671664.61510493882844Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC750412.583652555695494Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC228780.7876867734865142Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA156210.5378291410364908Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA88310.3040502621146694No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA83300.28680089269790465No Hit
CTGTAGGCACCATCAATAGATAGGAAGAGCACACGTCTGAACTCCAGTCA80050.27561118199840656Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACA76520.2634574346847979No Hit
AATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC68690.23649883936877636No Hit
CTGTAGGAACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA68210.2348462051731582Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGTAGGCACCATAAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA63790.21962819862184083Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG63010.21694266805396123No Hit
AATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG62170.21405055821162944No Hit
CGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC60040.2067169939685738Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGA58560.20162137186541773No Hit
ACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT57550.19814395407880447No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC55250.1902250818914674No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG55200.19005293249609048No Hit
CAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC50710.17459391679124545No Hit
TGGGAGAGGTCTCCGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA48900.1683621086786019No Hit
GCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA47320.16292218778469209No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG45120.15534761438810873No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCCGTCA44830.15434914789492277Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGGCACAATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA40480.13937215049713303Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGC40380.13902785170637924No Hit
CTGTAGGCACCATCAATAATCGGAAGAGCACACGTCTGAACTCCAGTCAC40100.13806381509226862Illumina Multiplexing Index Sequencing Primer (96% over 33bp)
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC37040.12752827209520273No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC35920.12367212563876033Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG35310.12157190301516224No Hit
CTGTAGGCACCATCAATAGATCGAAGAGCACACGTCTGAACTCCAGTCAC34200.11775018643779518Illumina Multiplexing Index Sequencing Primer (96% over 29bp)
CTGTAGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC33130.11406618937672967Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA32600.1122414057857346Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGGAGAGGTCTCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC32170.11076092098549332No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)