FastQCFastQC Report
Sun 2 Jul 2023
SRR3590734.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590734.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5153086
Total Bases257.6 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA625171.2131953551716388Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC372010.7219169251202094No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC287100.5571418757614369No Hit
ACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT190620.36991426108549325No Hit
TCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC126410.24530931562174585No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA122510.23774103517775563No Hit
AATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG118810.230560871679611No Hit
AAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACA118000.2289889980489361No Hit
TGCTAAGGCATTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC110130.21371659622990963No Hit
ATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT105160.20407189012564508No Hit
AATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG80680.156566375954137Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTAAGATCTGTTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC75590.1466887996823651No Hit
TGGGAGAGGTCTCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC70680.1371605286618543No Hit
TAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACA66960.12994155346912512No Hit
TCGAAATCTCTTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC64860.12586632553774574No Hit
AATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC62040.120393876601322No Hit
AAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT55770.10822641034906075Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGAGAGGTCTCCGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA51660.10025060711193252No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)