FastQCFastQC Report
Sun 2 Jul 2023
SRR3590735.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590735.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16639763
Total Bases831.9 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA875880.5263776893937733No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC331040.19894514122586962No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC290870.17480417239115725No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA290220.17441354182748878Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCAC260290.15642650679579992No Hit
CAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC241790.14530855998369688No Hit
TACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCAC222180.13352353636286765No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)