FastQCFastQC Report
Sun 2 Jul 2023
SRR3590736.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590736.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18045645
Total Bases902.2 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2670401.4798030217262945Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA328040.18178347185706026No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC204150.11312978837830401No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC181930.10081656820800809No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)