FastQCFastQC Report
Sun 2 Jul 2023
SRR3590737.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590737.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35632994
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA7647222.1461064989374736Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA1038740.2915107273893403No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC664980.18661917659795862No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC618730.17363963297611198No Hit
AATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC429780.1206129353037244No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)