FastQCFastQC Report
Sun 2 Jul 2023
SRR3590738.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590738.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11145471
Total Bases557.2 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACA818640.734504625241948No Hit
AATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC523470.4696705953476528No Hit
TCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT471810.4233199296826487No Hit
TAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACA423280.379777579610588No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC403570.3620932664039052No Hit
ACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCAC362470.32521730126972653No Hit
ACCTCGCATCCTTCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC236720.21239120356600452No Hit
AGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT234840.2107044197593803No Hit
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC234620.2105070301649881No Hit
ATCCCTTAGCTCTCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC226490.20321258742676734No Hit
TCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC215250.19312777360418415No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC189800.1702933864347231No Hit
ATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG177260.15904217955436786No Hit
TTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT147480.13232280627709678No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA125060.11220701215767373Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC118500.10632121334307003No Hit
TCCCGCTAGAGACCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC117950.10582773935708953No Hit
TTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC117110.10507406999668296No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC115260.1034142029529304No Hit
TGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG113370.10171844689201559No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)