FastQCFastQC Report
Sun 2 Jul 2023
SRR3590739.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590739.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15581947
Total Bases779 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA1588691.019570917549649No Hit
AATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1228160.7881941839488994No Hit
CAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC800810.5139344909849841No Hit
TCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC739220.47440797995269784No Hit
ATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT726900.46650139420959397No Hit
AATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC704840.4523439849975102No Hit
TCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT656000.42100002008734855No Hit
ACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT592040.3799525181288321No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA570280.36598763941373946Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCAC519170.33318686040967793No Hit
ATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG480930.30864563972653736No Hit
CACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCAC478340.30698345976918034No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC373100.23944376142467946No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC327640.21026897344728485No Hit
ATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC288670.18525926188813246No Hit
CAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACA256900.16487028225676803No Hit
TGTTTGAGCGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG182770.11729599645025106No Hit
TACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG181230.11630767323236306No Hit
TGGGAGAGGTCTCCGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA168240.1079711027126456No Hit
GTTTGAGCGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT158110.10146998959757725No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)