FastQCFastQC Report
Sun 2 Jul 2023
SRR3590740.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590740.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1996115
Total Bases99.8 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG1504247.535838366026006No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1394476.985920149891163Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG1336646.696207382841168No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA213801.0710805740150242No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG142290.7128346813685584No Hit
ACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG75550.37848520751559905Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG72740.36440786227246424No Hit
TCCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA69260.34697399698915143No Hit
AGAGGGGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG64510.3231777728237101No Hit
ATCCCTTAGCTCTCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC58730.29422152531292034No Hit
AATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC57970.29041412944644973No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG57740.28926189122370205No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA55630.27869135796284283No Hit
TCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT54810.27458337821217715No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT41190.20635083649990105No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC40520.20299431645972302No Hit
AAGAGGGGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG39550.19813487699856971No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC38520.1929748536532214No Hit
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC35160.17614215613829864No Hit
ATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGA35030.17549089105587604No Hit
ACCTCGCATCCTTCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC34060.17063145159472273No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGA31830.15945975056547343No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAG31370.15715527411997804No Hit
ATAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG31100.15580264664110036No Hit
AAGAGGTGCACAATCGACCTATCCTGACTGTAGGCACCATCAATAGATCG30860.15460031110432013No Hit
TAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC30470.15264651585705233No Hit
ATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG30370.15214554271672723No Hit
AAGATGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG29300.14678513011524885No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGGCTGAACTCCAGTCA28260.141575009455868Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
AAGAGGTGCACAATCGACCGATCCTGACTGGAGGCACCATCAATAGATCG27540.13796800284552746No Hit
AGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAA27290.13671556999471474No Hit
AAGAGGTGCACAATCGACCGATCCTTACTGTAGGCACCATCAATAGATCG27220.13636488879648717No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC26350.13200642247565897Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGAAGA26210.13130506007920387No Hit
AGAGGTGCACAATCGACCTATCCTGACTGTAGGCACCATCAATAGATCGG25640.12844951317935088No Hit
TCGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGA25590.12819902660918836No Hit
AAGAGGTGCACAATCGACAGATCCTGACTGTAGGCACCATCAATAGATCG24520.12283861400770998No Hit
ACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCAC23610.11827975843075172No Hit
AAGAGGCGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG23420.11732790946413407No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTATGCACCATCAATAGATCGG22890.11467275182041115No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGAGCG22140.11091545326797304No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTATGCACCATCAATAGATCG21400.10720825202956744No Hit
AGAGGTGCACAATCGACCGATCCTGACTGGAGGCACCATCAATAGATCGG21080.10560513798052718No Hit
AAGAGGTGCACAATCTACCGATCCTGACTGTAGGCACCATCAATAGATCG21060.10550494335246216No Hit
AGAGGTGCACAATCGACCGATCCTTACTGTAGGCACCATCAATAGATCGG20910.10475348364197455No Hit
AGAGGAGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG20900.10470338632794203No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGGCA20360.10199813137018658Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)