FastQCFastQC Report
Sun 2 Jul 2023
SRR3590741.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590741.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3832912
Total Bases191.6 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG48846612.743992035298488No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG46575012.151335590277053No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA40408310.542454405423344Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA684271.7852483959976122No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG592961.5470222118326744No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG296520.7736154652128722No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT266220.6945632980877202No Hit
TCCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA242680.6331478520769587No Hit
ACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG208300.5434510367052517Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC202820.5291538130799768No Hit
TCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT199450.5203615423469153No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA197150.5143608827961612No Hit
ATCCCTTAGCTCTCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC193510.5048641868114896No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG180480.47086914596526086No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAG176500.46048539596004295No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG156690.4088014543511565Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC154440.40293124392107094No Hit
AGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAA151620.3955739135153638No Hit
ATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGA144810.3778067432803049No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC134800.3516908293224577No Hit
ACCTCGCATCCTTCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC123150.3212961842066815No Hit
AAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGAAGA121680.3174609800590256No Hit
TAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC117610.30684242163660425No Hit
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC109290.2851356879573546No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGA105940.2763955968725606No Hit
ATAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG102620.26773377526016773No Hit
GAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGA99790.26035035503032683No Hit
TCGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGA91760.2394002262509549No Hit
ATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG91720.23929586695442No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC87000.22698146996330726Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGCAAGAGTTCGAATCTCTTAGCAACCACCACTGTAGGCACCATCAATAG86120.2246855654395405No Hit
ACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCAC85670.2235115233535234No Hit
TGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAG81030.21140584495548034No Hit
TTGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGATC78330.20436159243937768No Hit
TAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGA71590.1867770509732548No Hit
TAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACA69550.18145472684997724No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA69060.1801763254674253No Hit
AAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACA66170.1726363662927821No Hit
GTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGA65830.17174931227223583No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGA64510.16830545548658565No Hit
AAGAGGTGCACAATCGACCTATCCTGACTGTAGGCACCATCAATAGATCG62780.1637919159114532No Hit
AAGAGGTGCACAATCGACAGATCCTGACTGTAGGCACCATCAATAGATCG58150.1517123273375439No Hit
AGAGGTGCACAATCGACCTATCCTGACTGTAGGCACCATCAATAGATCGG56660.14782494354162057No Hit
TAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAG56380.14709442846587661No Hit
TGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAG55810.1456073084902549No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATA55790.1455551288419875No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT54780.14292005660448243No Hit
CTCGCCCCCGGGAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG54580.14239826012180817No Hit
AAGAGGTGCACAATCGACCGATCCTTACTGTAGGCACCATCAATAGATCG54510.14221563135287218No Hit
AGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAGA54470.14211127205633733No Hit
AAGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAG53460.13947619981883225No Hit
ATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGATC52340.13655413951585635No Hit
TTTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGA51480.134310414640357No Hit
AGAGGTGCACAATCGACCGATCCTTACTGTAGGCACCATCAATAGATCGG51210.1336059893887467No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC51190.1335538097404793Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGAGGTGCACAATCGACAGATCCTGACTGTAGGCACCATCAATAGATCGG51120.13337118097154332No Hit
CGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC50710.13230149818206105No Hit
ACAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG50490.13172752205111937No Hit
AGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGA50050.13057956978923596No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATC48810.12734443159665548No Hit
AGATGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG47340.12350922744899961No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGA44940.11724766965690839No Hit
TTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGA44240.11542138196754842No Hit
TAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGAAG41660.10869020734105037No Hit
AAGAGGTGCACAATCGACCGATACTGACTGTAGGCACCATCAATAGATCG41100.1072291771895624No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA40700.10618558422421387No Hit
TCTTCTTCATGATAAGACAGTTCTGTAGGCACCATCAATAGATCGGAAGA40210.10490718284166191No Hit
ATCCGGGTAGGACCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG39050.10188076324215115No Hit
ATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAG38580.10065454150786661No Hit
TCCCGCTAGAGACCACTGTAGGCACCATCAATAGATCGGAAGAGCACACG38500.1004458229147969No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)