FastQCFastQC Report
Sun 2 Jul 2023
SRR3590742.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590742.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11903802
Total Bases595.1 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT256990.2158890075624578No Hit
AATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG252690.21227671629618838Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC225280.18925045964306195No Hit
AATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG186750.1568826497618156No Hit
GATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACA167280.14052653093524237No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA166930.14023250722752278No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC146460.12303632066460782No Hit
GAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT143430.12049091542349243Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT140670.11817232847118929No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC131370.11035969852321133No Hit
CAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC125810.10568892190915137No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)