FastQCFastQC Report
Sun 2 Jul 2023
SRR3590743.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590743.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences889071
Total Bases44.4 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA30086433.840266975303436Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA241162.712494277734849No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG161351.814815689635586No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT122581.3787425301241407Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG86010.9674143009950836No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG85820.9652772388256956No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA69300.7794653070452191No Hit
TAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAG59010.6637265190294139No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA55640.6258217847618469No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA51850.5831930183303696No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT39670.4461960855769674No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC36760.4134652912984453Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGA32750.36836203182872906No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA31210.3510405805610575No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT30990.34856608752281876No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA25880.2910903628619087Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG24550.27613092767619235No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA23550.2648832320478342No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA23460.263870939441282No Hit
AATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG23330.2624087390095954Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT23110.2599342459713566No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT19950.2243915277857449No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA18920.212806401288536No Hit
TTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATCG18460.20763246129949126No Hit
TCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC18170.2043706295672674No Hit
ACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA16260.18288753091710336Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG15540.1747891900646855No Hit
TGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGC12610.14183344187359614No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC12570.14138353404846182Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT11800.13272280841462605No Hit
AATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG11620.13069822320152158No Hit
CTGTAGGCACCATCAATAGATCGAAGAGCACACGTCTGAACTCCAGTCAC11330.12743639146929772Illumina Multiplexing Index Sequencing Primer (96% over 29bp)
CTGTAGGCACCATCAATAGATAGGAAGAGCACACGTCTGAACTCCAGTCA11040.12417455973707386Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG10960.12327474408680521No Hit
TTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATCGG10910.1227123593053873No Hit
GAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAA10780.12125015887370075No Hit
ATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT10180.11450154149668587No Hit
CTGTAGGCACCATAAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA10020.11270191019614856Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA9940.11180209454587992No Hit
TTCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA9910.11146466367702916No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA9750.10966503237649187No Hit
AGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAG9110.10246650717434266No Hit
CTGTAGGCACCATCAATAATCGGAAGAGCACACGTCTGAACTCCAGTCAC8900.10010449109238745Illumina Multiplexing Index Sequencing Primer (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)